Plasmid-tagged in transgenic beans identifies a haploid-specific gene in biobalistic process.
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Genes introduced in plants are normally transferred to the progeny. However, transformants where the transgenes fails to segregate in a Medelian fashion may occur. These altered patterns of segregation could be due to different mechanisms, including insertional mutagenesis of genes that are essential to gametophyte development. Considering the difficulties encountered in isolation of gamethophytes, insertional mutagenesis becomes an important alternative for isolation of haploid-expressed genes. The rati onale of this approach is that mutations that affect gametophyte viability and function lead to a defect in the transmission of gametes. Particularly megagametophytic mutated genes reduce the number of seeds per pod. Pollens of transgenic bean plants were analyzed by Alexander and histochemical GUS assay, in order to determine their viability. Phenotypic analysis of female gametophyte mutants is facilitated by the fact that this kind of mutation results in transgenic plants with pods presenting a reduced number of seeds.
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Feijão transgênico, Biobalistic process, Plasmid, Haploid-specific