RESPUESTA DE PLANTAS IN VITRO DE ÑAME CLON ‘BLANCO DE GUINEA’ AL USO DEL PECTIMORF®
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Instituto Nacional de Ciencias Agrícolas
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The new growth bioregulators, as Pectimorf ® , they have been used in the in vitro propagation of different cultivations. Nevertheless, the effect of this substance has not been evaluated in the yam in vitro propagation, therefore, this investigation had as objective to determine the influence of the Pectimorf ® in the in vitro culture medium and its further effect in the plantlets acclimatization of yam ( Dioscorea rotundata Poir) clone "Blanco de guinea". The MS culture medium to 75 % with different concentrations of Pectimorf ® (3,0; 6,0; 9,0; 12,0; 15,0 mg L -1 ) and a control treatment (without Pectimorf ® ) were used. The further residual effect of the best treatment (6,0 mg L -1 ) was also evaluated in the plantlets acclimatization. To the 35 days it is selected 25 in vitro plants aleatorily to which were determined the following variables: roots number; greatest root length (cm), leaves number, bud novo number and shoot length (cm). After 45 days survival (%) in 70 plantlets is evaluated by treatment and it is selected in 40 plantlets by treatment to which were determined the following variables: buds number; shoot length (cm) and leaves number. The addition of Pectimorf ® 6,0 mg L -1 in the in vitro culture medium was the most appropriate with a significant influence in the in vitro plants vegetative development and a favorable residual effect in the acclimatized plantlets of yam clone "Blanco of guinea".
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