Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America

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dc.creatorNolt, Barry L.
dc.creatorVelasco, Ana Cecilia
dc.creatorPineda López, Benjamín
dc.date1991-02
dc.date2017-08-28T12:45:48Z
dc.date2017-08-28T12:45:48Z
dc.date.accessioned2026-06-27T14:43:00Z
dc.descriptionLarge quantities of cassava common mosaic virus (CCMV) were purified from systemically infected Nicotiana benthamiana plants. A polyclonal antiserum, with a titre of 1/128 in the tube precipitin test, was produced by immunising rabbits with purified virus. Viral antigens were detected in cassava, using both the double-antibody sandwich or plate-trapped antigen forms of enzyme-linked immunosorbent assay (ELISA). The virus reacted with antisera to the potexviruses potato virus X and tulip virus X in F(ab`)2 ELISA. As determined by ELISA, isolates of CCMV from cassava and chaya are closely serologically related to each other. Leaf extracts from infected N. benthamiana plants were infective to a dilution of 10(- 4) but not 10(-5); after heating for 10 min at 65 degrees C but not 70 degrees C; and after storage at room temperature for 14 days. The virus has a sedimentation coefficient of 126 S20,w, a single coat protein molecule of c. mol. wt 21 000, and a single-stranded RNA genome of c. mol. wt of 2.0 x 10(6). Several dsRNA species, including the putative viral replicative form of c. mol. wt 4.1 x 10(6), were isolated from virus-infected cassava and N. benthamiana. (AS)
dc.identifierhttps://hdl.handle.net/10568/83251
dc.identifier.urihttp://hdl.handle.net/123456789/86287
dc.languageen
dc.publisherWiley
dc.rightsLimited Access
dc.sourceNolt, B.L.; Velasco, A.C.; Pineda, B. 1991. Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America. Annals of Applied Biology 118(1):105-113.
dc.subjectmanihot esculenta
dc.subjectcassava common mosaic virus
dc.subjectpurification
dc.subjectelisa
dc.subjectserology
dc.subjectdiseases and pathogens
dc.subjectpests
dc.subjectstarch crops
dc.subjectviroses
dc.subjectvirus del mosaico comun de la yuca
dc.subjectpurificacion
dc.subjectserologia
dc.titleImproved purification procedure and some serological and physical properties of cassava common mosaic virus from South America
dc.typeJournal Article

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