Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes

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dc.creatorLee, Sang-Kyu
dc.creatorSong, Min-Young
dc.creatorSeo, Young-Su
dc.creatorKim, Hye-Kyung
dc.creatorKo, Seho
dc.creatorCao, Pei-Jian
dc.creatorSuh, Jung-Pil
dc.creatorYi, Gihwan
dc.creatorRoh, Jae-Hwan
dc.creatorLee, Sichul
dc.creatorAn, Gynheung
dc.creatorHahn, Tae-Ryong
dc.creatorWang, Guo-Liang
dc.creatorRonald, Pamela
dc.creatorJeon, Jong-Seong
dc.date2009-04-01
dc.date2024-12-19T12:55:59Z
dc.date2024-12-19T12:55:59Z
dc.date.accessioned2026-06-27T04:10:41Z
dc.descriptionRice blast, caused by the fungus Magnaporthe oryzae, is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to M. oryzae, we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M. oryzae. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to M. oryzae. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M. oryzae.
dc.identifierhttps://hdl.handle.net/10568/166192
dc.identifier.urihttp://hdl.handle.net/123456789/24077
dc.languageen
dc.publisherOxford University Press
dc.sourceLee, Sang-Kyu; Song, Min-Young; Seo, Young-Su; Kim, Hye-Kyung; Ko, Seho; Cao, Pei-Jian; Suh, Jung-Pil; Yi, Gihwan; Roh, Jae-Hwan; Lee, Sichul; An, Gynheung; Hahn, Tae-Ryong; Wang, Guo-Liang; Ronald, Pamela and Jeon, Jong-Seong. 2009. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes.
dc.subjectcrosses
dc.subjectdisease resistance
dc.subjectfungal diseases
dc.subjectgenes
dc.subjectgene expression
dc.subjectgenetic transformation
dc.subjectgenomics
dc.subjectleucine
dc.subjectnucleotides
dc.subjectinduced resistance
dc.subjecttransgenic rice
dc.titleRice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)