Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.)

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dc.creatorHussain, Appibhai J.
dc.creatorAli, Jauhar
dc.creatorSiddiq, Ebrahimali A.
dc.creatorGupta, Vidya S.
dc.creatorReddy, Umesh K.
dc.creatorRanjekar, Prabhakar K.
dc.date2012-02
dc.date2024-12-19T12:55:33Z
dc.date2024-12-19T12:55:33Z
dc.date.accessioned2026-06-27T04:16:05Z
dc.descriptionWith 3 figuresGenetic analysis of F2 and backcross populations of an induced temperature‐sensitive genic male sterility (TGMS) mutant source F 61 with normal pollen parents revealed that TGMS trait was controlled by a single recessive gene. Molecular tagging of TGMS trait was attempted using polymorphic randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers through bulked segregant analysis. The RAPD primers UBC 345830, UBC 313927, microsatellites RM224 and RM21 produced putative markers, which differentiate parents and bulks from sterile parent and sterile bulks. The RAPD analysis of individual F2 plants with the primer UBC345830 showed perfect marker–phenotype cosegregation. The 830‐bp RAPD fragment, which segregated with TGMS locus at a distance of 1.33 cM, was eluted and cloned, and sequence information was used for designing sequence‐characterized amplified region (SCAR) primer, which cosegregated with TGMS locus at a distance of 0.8 cM. TGMS locus was mapped onto chromosome 11 using RM21 and RM224, flanking it at a distance of 4.3 and 3.0 cM, respectively. The DNA markers tightly linked to TGMS gene (tms8) in F 61 can be cost effectively used for marker‐assisted selection of TGMS trait.
dc.identifierhttps://hdl.handle.net/10568/165861
dc.identifier.urihttp://hdl.handle.net/123456789/27129
dc.languageen
dc.publisherWiley
dc.sourceHussain, Appibhai J.; Ali, Jauhar; Siddiq, Ebrahimali A.; Gupta, Vidya S.; Reddy, Umesh K. and Ranjekar, Prabhakar K. 2012. Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.). Plant Breeding, Volume 131 no. 1 p. 42-47
dc.subjectbackcrossing
dc.subjectdna
dc.subjectgenes
dc.subjectgenetic analysis
dc.subjectgenetic distance
dc.subjectgenetic markers
dc.subjectmale sterility
dc.subjectmicrosatellites
dc.subjectmolecular genetics
dc.subjectmutants
dc.subjectmutations
dc.subjectplant breeding
dc.subjectpollen
dc.subjectrapd
dc.subjectrecessive genes
dc.subjectselection
dc.subjectsimple sequence repeats
dc.subjectsterility
dc.titleMapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.)
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)