A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate

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dc.creatorShan, J.
dc.creatorToye, Philip G.
dc.date2009-06-30
dc.date2010-05-11T17:13:53Z
dc.date2010-05-11T17:13:53Z
dc.date.accessioned2026-06-27T17:10:44Z
dc.descriptionWe have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immuno-PCR is reproducible, with a larger dynamic range and a sensitivity several orders of magnitude greater than the corresponding conventional ELISA. The minimum concentration of analyte detected was of the order of 1 pg/mL These characteristics should contribute to the adoption of immuno-PCR by research and clinical laboratories.
dc.identifierhttps://hdl.handle.net/10568/1494
dc.identifier.urihttp://hdl.handle.net/123456789/144547
dc.languageen
dc.publisherInforma UK Limited
dc.rightsLimited Access
dc.sourceShan, J.; Toye, P.G. 2009. A novel immuno-polymerase chain reaction protocol incorporating a highly purified streptavidin-DNA conjugate. Journal of Immunoassay and Immunochemistry 30:322-337.
dc.titleA novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)