Development, characterization, validation, and mapping of SSRs derived from Theobroma cacao L.-Moniliophthora perniciosa interaction ESTs.

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dc.contributorLÍVIA SANTOS LIMA, COMISSÃO EXECUTIVA DO PLANO DA LAVOURA CACAUEIRA
dc.contributorKARINA PERES GRAMACHO, COMISSÃO EXECUTIVA DO PLANO DA LAVOURA CACAUEIRA
dc.contributorJOSÉ LUIS PIRES, COMISSÃO EXECUTIVA DO PLANO DA LAVOURA CACAUEIRA
dc.contributorDIDIER CLEMENT, CENTRE DE COOPERATION INTERNATIONALE EN RECHERCHE AGRONOMIQUE POUR LE DÉVELOPPEMENT
dc.contributorUILSON VANDERLEI LOPES, COMISSÃO EXECUTIVA DO PLANO DA LAVOURA CACAUEIRA
dc.contributorNICOLAS CARELS, UNIVERSIDADE ESTADUAL DE SANTA CRUZ
dc.contributorABELMON DA SILVA GESTEIRA, CNPMF
dc.contributorFERNANDA AMATO GAIOTTO, UNIVERSIDADE ESTADUAL DE SANTA CRUZ
dc.contributorJÚLIO CÉZAR DE MATTOS CASCARDO, UNIVERSIDADE ESTADUAL DE SANTA CRUZ
dc.contributorFABIENNE MICHELI, CENTRE DE COOPERATION INTERNATIONALE EN RECHERCHE AGRONOMIQUE POUR LE DÉVELOPPEMENT.
dc.creatorLIMA, L. S.
dc.creatorGRAMACHO, K. P.
dc.creatorPIRES, J. L.
dc.creatorCLEMENT, D.
dc.creatorLOPES, U. V.
dc.creatorCARELS, N.
dc.creatorGESTEIRA, A. da S.
dc.creatorGAIOTTO, F. A.
dc.creatorCASCARDO, J. C. de MATTOS
dc.creatorMICHELI, F.
dc.date2022-08-16T13:28:17Z
dc.date2022-08-16T13:28:17Z
dc.date2010-07-21
dc.date2010
dc.date.accessioned2026-07-07T04:51:04Z
dc.descriptionIn this study, we report results of the detection and analysis of SSR markers derived of cacao?Moniliophthora perniciosa expressed sequence tags (ESTs) in relation to cacao resistance to witches? broom disease (WBD), and we compare the polymorphism of those ESTs (EST-simple sequence repeat (SSR)) with classical neutral SSR markers. A total of 3,487 ESTs was used in this investigation. SSRs were identified in 430 sequences: 277 from the resistant genotype TSH 1188 and 153 from the susceptible one Catongo, totalizing 505 EST-SSRs with three types of motifs: dinucleotides (72.1%), trinucleotides (27.3%), and tetranucleotides (0.6%). EST-SSRs were classified into 16 main categories; most of the EST-SSRs belonged to ?Unknown function? and ?No homology? categories (45.82%). A high frequency of SSRs was found in the 5?UTR and in the ORF (about 27%) and a low frequency was observed in the 3?UTR (about 8%). Forty-nine ESTSSR primers were designed and evaluated in 21 cacao accessions, 12 revealed polymorphism, having 47 alleles in total, with an average of 3.92 alleles per locus. On the other hand, the 11 genomic SSR markers revealed a total of 47 alleles, with an average of 5.22 alleles per locus. The association of EST-SSR with the genomic SSR enhanced the analysis of genetic distance among the genotypes. Among the 12 polymorphic EST-SSR markers, two were mapped on the F2 Sca 6×ICS 1 population reference for WBD resistance.
dc.identifierTree Genetics & Genomes, Heidelberg, v. p. 663–676mar. 2010.
dc.identifier1614-2950 (Online)
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/858299
dc.identifier10.1007/s11295-010-0282-1
dc.identifier.urihttp://hdl.handle.net/123456789/473306
dc.languageeng
dc.rightsopenAccess
dc.subjectMicrosatellite
dc.subjectResistance
dc.subjectGenetic diversity
dc.subjectTheobroma cacao L
dc.subjectCacau
dc.subjectDoença de Planta
dc.subjectMarcador Molecular
dc.subjectVassoura de Bruxa
dc.subjectwitches' broom
dc.titleDevelopment, characterization, validation, and mapping of SSRs derived from Theobroma cacao L.-Moniliophthora perniciosa interaction ESTs.
dc.typeArtigo de periódico

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Embrapa Yuca y Frutas Tropicales (CNPMF)