Preservation and maceration of Amazon açai leaflet tissue to obtain genomic DNA.

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dc.contributorHELLEN SANDRA FREIRES DA SILVA AZÊVEDO, FUNDAÇÃO OSWALDO CRUZ
dc.contributorPOLINAR BANDEIRA RUFINO, FACULDADE META
dc.contributorJOSÉ MARLO ARAÚJO DE AZEVEDO, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO ACRE
dc.contributorLUCIELIO MANOEL DA SILVA, CPAF-AC
dc.contributorLUCIA HELENA DE OLIVEIRA WADT, CPAF-RO
dc.contributorTATIANA DE CAMPOS, CPAF-AC.
dc.creatorAZÊVEDO, H. S. F. da S.
dc.creatorRUFINO, P. B.
dc.creatorAZEVEDO, J. M. A. de
dc.creatorSILVA, L. M. da
dc.creatorWADT, L. H. de O.
dc.creatorCAMPOS, T. de
dc.date2020-01-22T00:37:56Z
dc.date2020-01-22T00:37:56Z
dc.date2020-01-21
dc.date2019
dc.date2020-01-27T11:11:11Z
dc.date.accessioned2026-06-30T22:52:05Z
dc.descriptionThe objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E. precatoria were collected in an experimental field at Embrapa Acre, Brazil. The study was conducted in a completely randomized design with 10 replicates, in a 12 × 2 factorial structure, with 12 storage treatments (fresh; lyophiliser 3 days; refrigerator 3, 5, and 7 days; silica gel 7, 10, 20, and 30 days; and transport buffer 3, 5, and 7 days) and two leaf tissue maceration methods (liquid nitrogen and the TissueLyser®). Statistically significant differences in the obtained DNA concentration were found between the maceration and storage treatments. The TissueLyser® macerator produced higher DNA concentrations when compared to liquid nitrogen. For the storage treatments, five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen. The DNA concentrations ranged from 285.00 ng/uL (7 days in transport buffer) to 702.00 ng/uL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/uL (30 days in silica gel) to 2,850.00 ng/uL (3 days in lyophiliser) using the TissueLyser® macerator. The DNA purity (A260/A280 nm) varied from 1.30 to 1.70 when the leaflets were macerated with liquid nitrogen and from 1.30 to 1.90 with the TissueLyser® macerator. Despite the variations in leaf tissue preservation and DNA concentration, all treatments were effective for DNA isolation and it was possible to amplify genomic regions of microsatellite markers by PCR. It was concluded that leaflets of E. precatoria stored in a lyophiliser and processed with an automatic macerator resulted in satisfactory DNA for molecular studies.
dc.identifierBioscience Journal, v. 35, n. 4, p. 1188-1197, jul./ago. 2019.
dc.identifier1981-3163
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1119144
dc.identifier10.14393/BJ-v35n4a2019-42190
dc.identifier.urihttp://hdl.handle.net/123456789/369855
dc.languageeng
dc.rightsopenAccess
dc.subjectAmazonia Occidental
dc.subjectHojas
dc.subjectExtracción
dc.subjectMaceración
dc.subjectEmbrapa Acre
dc.subjectRio Branco
dc.subjectAcre
dc.subjectAmazônia Ocidental
dc.subjectWestern Amazon
dc.subjectLeaflet
dc.subjectAçaí
dc.subjectCampo Experimental
dc.subjectMaceração
dc.subjectFolha
dc.subjectExtração
dc.subjectDNA
dc.subjectExtraction
dc.subjectLeaves
dc.subjectEuterpe precatoria
dc.subjectMaceration
dc.titlePreservation and maceration of Amazon açai leaflet tissue to obtain genomic DNA.
dc.typeArtigo de periódico

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