A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome

Vista sencilla de ítem
dc.creatorGotarkar, Dhananjay
dc.creatorLongkumer, Toshisangba
dc.creatorYamamoto, Naoki
dc.creatorNanda, Amrit Kaur
dc.creatorIglesias, Tamara
dc.creatorLi, Lin-Feng
dc.creatorMiro, Berta
dc.creatorGonzalez, Elisa Blanco
dc.creatorBayon, Maria Montes
dc.creatorOlsen, Kenneth M.
dc.creatorHsing, Yue-Ie Caroline
dc.creatorKohli, Ajay
dc.date2021-08
dc.date2024-12-19T12:53:43Z
dc.date2024-12-19T12:53:43Z
dc.date.accessioned2026-06-27T04:15:58Z
dc.descriptionDrought stress in plants causes differential expression of numerous genes. One of these differentially expressed genes in rice is a specific amidohydrolase. We characterized this amidohydrolase gene on the rice chromosome 12 as the first plantguanine deaminase(OsGDA1). The biochemical activity of GDA is known from tea and coffee plants where its catalytic product, xanthine, is the precursor for theine and caffeine. However, no plant gene that is coding for GDA is known so far. RecombinantOsGDA1converted guanine to xanthine in vitro. Measurement of guanine and xanthine contents in theOsGDA1knockout (KO) line and in the wild type Tainung 67 rice plants also suggested GDA activity in vivo. The content of cellular xanthine is important because of its catabolic products allantoin, ureides, and urea which play roles in water and nitrogen stress tolerance among others. The identification ofOsGDA1fills a critical gap in the S‐adenosyl‐methionine (SAM) to xanthine pathway. SAM is converted to S‐adenosyl‐homocysteine (SAH) and finally to xanthine. SAH is a potent inhibitor of DNA methyltransferases, the reduction of which leads to increased DNA methylation and gene silencing in Arabidopsis. We report that theOsGDA1KO line exhibited a decrease in SAM, SAH and adenosine and an increase in rice genome methylation. The OsGDA1 protein phylogeny combined with mutational protein destabilization analysis suggested artificial selection for null mutants, which could affect genome methylation as in the KO line. Limited information on genes that may affect epigenetics indirectly requires deeper insights into such a role and effect of purine catabolism and related genetic networks.
dc.identifierhttps://hdl.handle.net/10568/164306
dc.identifier.urihttp://hdl.handle.net/123456789/27070
dc.languageen
dc.publisherWiley
dc.rightsOpen Access
dc.sourceGotarkar, D., Longkumer, T., Yamamoto, N., Nanda, A.K., Iglesias, T., Li, Lin‐Feng. Miro, B., Gonzalez, E.B., Bayon, M.M., Olsen, K.M., Hsing, Y.‐I.C. and Kohli, A. 2021. A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome. Physiologia Plantarum, Volume 172 no. 4 p. 1853-1866
dc.subjectcell biology
dc.subjectgeneral medicine
dc.subjectgenetics physiology
dc.subjectplant science
dc.titleA drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome
dc.typeJournal Article

Archivos

Colecciones

Instituto Internacional de Investigación del Arroz (IRRI)