Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1.

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dc.creatorFuentes, S.
dc.date2022-10
dc.date2023-02-06T19:59:12Z
dc.date2023-02-06T19:59:12Z
dc.date.accessioned2026-06-27T18:00:11Z
dc.descriptionThis standard operational procedure (SOP) for LAMP testing covers the steps on how to use a field-deployable LAMP assay in the diagnosis of the major viruses infecting potato worldwide: Potato leaf roll virus (PLRV), Potato virus Y (PVY), and Potato virus X (PVX). It includes different steps from collecting samples, processing of them for nucleic acid extraction, preparing LAMP reaction, setting the Genie instrument, and recording of results. LAMP test can be rapidly adopted to detect different pathogens in different plants by just replacing the specific primers (for those pathogens) in the reaction, which makes it broadly applicable and adjustable to new pathogens.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/128477
dc.identifier.urihttp://hdl.handle.net/123456789/152087
dc.languageen
dc.publisherInternational Potato Center
dc.rightsOpen Access
dc.sourceFuentes, S. 2022. Loop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1. International Potato Center. ISBN: 978-92-9060-603-1. 14 p.
dc.subjectpotatoes
dc.subjectviroses
dc.subjectplant viruses
dc.subjectdetection
dc.subjectdiagnosis
dc.titleLoop-mediated Isothermal Amplification (LAMP): Potato virus detection CIP – SOPXXX, v.1.1.
dc.typeManual

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