Prevalence and antimicrobial resistance in enterococci isolated from semi-intensive and free-range poultry farms in peri-urban Wakiso and rural Soroti, Uganda

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dc.creatorOchieng, Linnet
dc.creatorAyebare, Dreck
dc.creatorNdoboli, Dickson
dc.creatorMbatidde, Irene
dc.creatorTenhagen, B.-A.
dc.creatorRoesel, Kristina
dc.creatorWampande, E.
dc.creatorMoodley, Arshnee
dc.date2025-09
dc.date2025-08-22T11:35:14Z
dc.date2025-08-22T11:35:14Z
dc.date.accessioned2026-06-27T17:19:38Z
dc.description<i>Enterococcus</i> spp. are commensal bacteria with increasing clinical relevance due to their role in antimicrobial resistance (AMR). This was a cross-sectional study conducted on broiler chickens in the Soroti and Wakiso districts. It assessed the prevalence, species distribution, AMR profiles, and the presence of mutations in the <i>gyrA</i> gene in antibiotic-resistant enterococci spp. A total of 402 environmental samples were collected, and 75% (303/402) tested positive for <i>Enterococcus</i> spp. The most frequently isolated species were <i>E. faecalis</i> (33.1%), followed by <i>E. faecium</i> (21.4%) and <i>E. lactis</i> (13.4%). Phenotypic resistance was observed to sulfamethoxazole-trimethoprim (21.2%), fluoroquinolone (11.9%), tigecycline (11.8%), ampicillin (4.1%), glycopeptides (2.3%), gentamicin (2.0%), and linezolid (1.0%). Resistance to fluoroquinolones, tigecycline, and sulfamethoxazole-trimethoprim was significantly associated with the semi-intensive system (Fisher’s exact p < 0.001). Only 3.3% of the isolates were multidrug-resistant. Eleven acquired resistance genes were detected, with <i>tetL</i> and <i>tetM</i> showing significant distribution differences between systems. Mutations in <i>gyrA</i> associated with fluoroquinolone resistance were also identified. While overall AMR levels were low, the detection of resistance to critically important antibiotics not approved for use in poultry highlights potential public health risks. These findings underscore the importance of routine AMR surveillance in poultry. It also highlights the need for deeper investigation into its role in AMR transmission and strategic interventions to limit the development of resistance in food animal production.
dc.identifierhttps://hdl.handle.net/10568/176178
dc.identifier.urihttp://hdl.handle.net/123456789/148970
dc.languageen
dc.publisherElsevier
dc.rightsOpen Access
dc.sourceOchieng, L., Ayebare, D., Ndoboli, D., Mbatidde, I., Tenhagen, B.-A., Roesel, K., Wampande, E. and Moodley, A. 2025. Prevalence and antimicrobial resistance in enterococci isolated from semi-intensive and free-range poultry farms in peri-urban Wakiso and rural Soroti, Uganda. The Microbe 8: 100515.
dc.subjectantimicrobial resistance
dc.subjectpoultry
dc.titlePrevalence and antimicrobial resistance in enterococci isolated from semi-intensive and free-range poultry farms in peri-urban Wakiso and rural Soroti, Uganda
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)