Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance

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dc.creatorOuso, D.O.
dc.creatorOtiende, M.Y.
dc.creatorJeneby, M.M.
dc.creatorOundo, J.W.
dc.creatorBargul, J.L.
dc.creatorMiller, S.E.
dc.creatorWambua, Lillian
dc.creatorVillinger, J.
dc.date2020-03-16
dc.date2020-05-01T12:49:14Z
dc.date2020-05-01T12:49:14Z
dc.date.accessioned2026-06-27T17:00:02Z
dc.descriptionReliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) ‘barcode’ gene, which remains costly for purposes of screening large numbers of unknown samples during routine surveillance. Here, we adapted a rapid, low-cost approach to differentiate 10 domestic and 24 wildlife species that are common in the East African illegal wildlife products trade based on their unique high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR products. Using the approach, we identified (i) giraffe among covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences among savannah elephant reference samples. This approach is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines.
dc.identifierhttps://hdl.handle.net/10568/108124
dc.identifier.urihttp://hdl.handle.net/123456789/139286
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceOuso, D.O., Otiende, M.Y., Jeneby, M.M., Oundo, J.W., Bargul, J.L., Miller, S.E., Wambua, L. and Villinger, J. 2020. Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance. Scientific Reports 10: 4741.
dc.subjectbiodiversity
dc.subjectresearch
dc.subjectpcr
dc.subjectwildlife
dc.subjectforensic sciences
dc.titleThree-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)