Determination of neonicotinoids and other residues in orange pollen by micro-QuEChERS and UHPLC–MS/MS.

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dc.contributorGABRIELA BRITO ALMEIDA, UNIVERSIDADE ESTADUAL DE CAMPINAS; JORDANA ALVES FERREIRA; ROBSON ROLLAND MONTICELLI BARIZON, CNPMA; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; CARLA BEATRIZ GRESPAN BOTTOLI, UNIVERSIDADE ESTADUAL DE CAMPINAS.
dc.creatorALMEIDA, G. B.
dc.creatorFERREIRA, J. A.
dc.creatorBARIZON, R. R. M.
dc.creatorQUEIROZ, S. C. do N. de
dc.creatorBOTTOLI, C. B. G.
dc.date2025-03-10T12:29:06Z
dc.date2025-03-10T12:29:06Z
dc.date2025-03-10
dc.date2025
dc.date.accessioned2026-07-07T05:10:30Z
dc.descriptionAbstract:Producers of citrus crops use different types of insecticides, such as neonicotinoids, to combat pests and diseases of these cultures. Neonicotinoids are systemic insecticides and are translocated to the plant tissue, which may leave residues in pollen and cause adverse effects on bees. Pesticide contamination of pollen is also a food safety issue in human nutrition since the product is consumed as a food supplement. Therefore, the determination of these pesticides in pollen is important to help understand their effects on bees because pollen is their main nutrient source. In this work, we developed and validated a method using UHPLC–MS/MS to determine pesticide residues in orange pollen using micro-QuEChERS extraction, which uses low consumption of solvent, sample, and reagents. The analytical method demonstrated linearity over the range of 50 to 1000 μg kg−1, with a correlation coefficient of ≥0.990. Trueness and precision were assessed using spiked samples, analyzed in quintuplicate at concentrations of 50, 100, and 200 μg kg−1. Trueness values ranged between 81 and 115 %, while precision, expressed as relative standard deviation (RSD), was consistently below 20 %. The limit of quantification (LOQ) was established at 50 μg kg−1, with acceptable precision and accuracy observed at this level. Experimental treatment samples showed high concentrations of clothianidin, imidacloprid, and thiamethoxam, while in commercial samples, the analytes were below the LOQ or not detected.
dc.identifierGreen Analytical Chemistry, v. 12, n., article 100223, 2025. 2772-5774
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1173703
dc.identifierhttps://doi.org/10.1016/j.greeac.2025.100223
dc.identifier.urihttp://hdl.handle.net/123456789/482935
dc.languageeng
dc.rightsopenAccess
dc.subjectSystemic insecticides
dc.subjectRepresentative sampling
dc.subjectInseticida Sistêmico
dc.subjectResíduo Quimico
dc.subjectPólen
dc.subjectLaranja
dc.subjectAnálise Química
dc.subjectCromatografia
dc.subjectEspectrometria
dc.subjectPesticide residues
dc.subjectNeonicotinoid insecticides
dc.subjectBee pollen
dc.subjectCitrus
dc.subjectTandem mass spectrometry
dc.subjectLiquid chromatography
dc.subjectColony collapse disorder
dc.titleDetermination of neonicotinoids and other residues in orange pollen by micro-QuEChERS and UHPLC–MS/MS.
dc.typeArtigo de periódico

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