Sulfuric acid as a germination stimulator in forage soybean seeds (Neonotonia wightii)
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Universidad Nacional de Colombia
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Forage soybean (Neonotonia wightii) is a legume frequently used as a cover crop in field crops. However, as a species of the Phaseoloideae subfamily, it is characterized by the physical latency of the seed, which makes it difficult to obtain a high, homogeneous, and rapid germination. The aim of the present study was to determine which method of scarification stimulates the germination process to a greater extent. The following immersion times were evaluated: 5 to 20 minutes in 98% sulfuric acid, 24 hours in 5.4% sodium hypochlorite, water, and gibberellic acid 100 ppm, 15 minutes of treatment with sandpaper and the combination of these last two. They were distributed in a completely randomized experimental design, where eight treatments and a control were evaluated, with three replicates each and 200 seeds as experimental units, subjected to controlled conditions (germination chambers at constant temperatures and a relative humidity of 27 °C and 70%, respectively) during the spring of 2022. The results were favorable, a high germination percentage was obtained from seeds subjected to a 20-minute immersion time in sulfuric acid (90% germination and an average of six germinated seeds per day) and this was significantly higher than the rest, making it an effective method to break the physical latency of the seeds, as it removes the impermeable cover allowing inhibition and consequently the activation of the seed's metabolism.
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