Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus

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dc.creatorEdwards, Anne
dc.creatorNjaci, Isaac
dc.creatorSarkar, Abhimanyu
dc.creatorJiang, Zhouqian
dc.creatorKaithakottil, Gemy George
dc.creatorMoore, Christopher
dc.creatorCheema, Jitender
dc.creatorStevenson, Clare E.M.
dc.creatorRejzek, Martin
dc.creatorNovák, Petr
dc.creatorVigouroux, Marielle
dc.creatorVickers, Martin
dc.creatorWouters, Roland H.M.
dc.creatorPaajanen, Pirita
dc.creatorSteuernagel, Burkhard
dc.creatorMoore, Jonathan D.
dc.creatorHiggins, Janet
dc.creatorSwarbreck, David
dc.creatorMartens, Stefan
dc.creatorKim, Colin Y.
dc.creatorWeng, Jing-Ke
dc.creatorMundree, Sagadevan
dc.creatorKilian, Benjamin
dc.creatorKumar, Shiv
dc.creatorLoose, Matt
dc.creatorYant, Levi
dc.creatorMacas, Jiří
dc.creatorWang, Trevor L.
dc.creatorMartin, Cathie
dc.creatorEmmrich, Peter M.F.
dc.date2023-02-16
dc.date2023-03-10T14:38:58Z
dc.date2023-03-10T14:38:58Z
dc.date.accessioned2026-06-27T14:24:31Z
dc.descriptionGrass pea ( Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.
dc.identifierhttps://hdl.handle.net/10568/129553
dc.identifier.urihttp://hdl.handle.net/123456789/78826
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceEdwards, Anne; Njaci, Isaac; Sarkar, Abhimanyu; Jiang, Zhouqian; Kaithakottil, Gemy George; Moore, Christopher; Cheema, Jitender; Stevenson, Clare E.M.; Rejzek, Martin; Novák, Petr; Vigouroux, Marielle; Vickers, Martin; Wouters, Roland H.M.; Paajanen, Pirita; Steuernagel, Burkhard; Moore, Jonathan D.; Higgins, Janet; Swarbreck, David; Martens, Stefan; Kim, Colin Y.; Weng, Jing-Ke; Mundree, Sagadevan; Kilian, Benjamin; Kumar, Shiv; Loose, Matt; Yant, Levi; Macas, Jiří; Wang, Trevor L.; Martin, Cathie; Emmrich, Peter M.F. 2023. Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus. Nature Communications 14:
dc.subjectgenomics
dc.subjectbiosynthesis
dc.subjectlathyrus
dc.titleGenomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus
dc.typeJournal Article

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Centro Internacional para la Investigación Agrícola en las Áreas Secas (ICARDA)