Ligninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.

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dc.contributorMUHAMMAD FERHAN, University of Toronto; ALCIDES LOPES LEÃO, UNESP; ITAMAR SOARES DE MELO, CNPMA; N. YAN, University of Toronto; M. SAIN, University of Toronto.
dc.creatorFERHAN, M.
dc.creatorLEÃO, A. L.
dc.creatorMELO, I. S. de
dc.creatorYAN, N.
dc.creatorSAIN, M.
dc.date2012-12-20T11:11:11Z
dc.date2012-12-20T11:11:11Z
dc.date2012-12-20
dc.date2012
dc.date2015-04-10T11:11:11Z
dc.date.accessioned2026-07-07T04:55:48Z
dc.descriptionABSTRACT: The potential of ligninases as a green tool for effective valorization of lignin can be shown through enzymatic cocktails containing different lignin degrading enzymes. The present study deals with the screening of potential fungal strains useful for the liquefaction of bark containing lignin. Three different local isolates (Pleurotus ostreatus POS97/14, Pycnoporus sanguineus and the local isolated fungal strain) were selected out of ten different strains for ligninases production. Maximum production of enzymes was observed in the local isolated fungal strain after ten days in submerged fermentation. The isolated fungal strain produces ligninases mainly for manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related phenols. Furthermore, the partial purification for MnP was determined by FPLC and the molecular weight was evaluated by SDS-PAGE.
dc.identifierFermentation Technology, v. 1, n. 5, 2012.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/943353
dc.identifierhttp://dx.doi.org/10.4172/2167-7972.1000106
dc.identifier.urihttp://hdl.handle.net/123456789/474835
dc.languageeng
dc.rightsopenAccess
dc.subjectLigninases
dc.subjectFungal strain
dc.subjectP sanguineus
dc.subjectLcc
dc.subjectFast protein
dc.subjectFungo
dc.subjectPeroxidase
dc.subjectLignina
dc.subjectLignin peroxidase
dc.subjectFungi
dc.subjectPleurotus ostreatus
dc.subjectManganese peroxidase
dc.subjectliquid chromatography
dc.subjectsubmerged fermentation
dc.titleLigninases production and partial purification of mnp from brazilian fungal isolate in submerged fermentation.
dc.typeArtigo de periódico

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