Cultivo de tejidos

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dc.creatorInternational Center for Tropical Agriculture
dc.date1981
dc.date2017-06-20T08:51:06Z
dc.date2017-06-20T08:51:06Z
dc.date.accessioned2026-06-27T14:38:43Z
dc.descriptionDuring 1981, the efforts to use meristem-culture methods have continued, together with thermotherapy for recovering healthy clones. Frog skin disease was eradicated up to 100 percent in different cassava clones during various propagation cycles through the culture of small meristem tips. A routine "cleaning-up" program consists of: (1) disinfestation of stakes containing dormant buds and treatment with thermotherapy; (2) cutting and disinfestation of shoot terminal buds; (3) isolation of meristem tips (0.5-0.6 mm) under sterile conditions and planting in culture medium; (4) transfer of meristem tips and nodes grown from the meristem to a rooting medium; (5) hardening of rooted plantlets prior to potting; (6) transplant to the field and to the greenhouse; (7) evaluation of the presence of frog skin disease after 3 mo. of growth; and (8) selection of symptom-free plants, grown again for 3 mo., and reevaluated. A germplasm bank in vitro is a long-term goal to conserve material and to isolate microorganisms. The main steps and procedures followed in the international exchange of clonal materials are described. During 1981, work was initiated aimed atdeveloping anther-culture methods for cassava and to observe microsporogenesis stages in relation to the size of the floral bud. (CIAT)
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/81630
dc.identifier.urihttp://hdl.handle.net/123456789/84233
dc.languagees
dc.publisherInternational Center for Tropical Agriculture
dc.rightsOpen Access
dc.sourceCultivo de tejidos.1981. In: Centro Internacional de Agricultura Tropical. Programa de Yuca: Informe Anual 1981 . CIAT, Cali, CO. p. 115-125.
dc.subjectmanihot esculenta
dc.subjectapical meristems
dc.subjectafrican cassava mosaic virus
dc.subjectdevelopment
dc.subjectdisease control
dc.subjectgermplasm
dc.subjectpest control
dc.subjectsoybean flour
dc.subjecttissue culture
dc.subjectdiseases and pathogens
dc.subjectflours
dc.subjectpests
dc.subjectplant tissues
dc.subjectviroses
dc.subjectmeristemas apicales
dc.subjectvirus de mosaico de yuca
dc.subjectdesarrollo
dc.subjectcontrol de enfermedades
dc.subjectgermoplasma
dc.subjectcontrol de plagas
dc.subjectharina de soya
dc.subjectcultivo de tejidos
dc.titleCultivo de tejidos
dc.typeBook Chapter

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Centro Internacional de Agricultura Tropical (CIAT)