Liquid culture production of microsclerotia and submerged conidia by Trichoderma harzianum active against damping-off disease caused by Rhizoctonia solani.

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dc.contributorNILCE N. KOBORI, USDA; GABRIEL MOURA MASCARIN, CNPAF; MARK A. JACKSON, USDA; DAVID A. SCHISLER, USDA.
dc.creatorKOBORI, N. N.
dc.creatorMASCARIN, G. M.
dc.creatorJACKSON, M. A.
dc.creatorSCHISLER, D. A.
dc.date2017-08-09T23:47:32Z
dc.date2017-08-09T23:47:32Z
dc.date2015-03-19
dc.date2015
dc.date2017-08-09T23:47:32Z
dc.date.accessioned2026-07-01T00:03:06Z
dc.descriptionMedia and culturing protocols were identified that supported the formation of submerged conidia and microsclerotia (MS) by Trichoderma harzianum Rifai strain T-22 using liquid culture fermentation. Liquid media with a higher carbon concentration (36 g L -1) promoted MS formation at all C:N ratios tested. Hyphae aggregated to form MS after 2 d growth and after 7 d MS were fully melanized. This is the first report of MS formation by T. harzianum or any species of Trichoderma. Furthermore, submerged conidia formation was induced by liquid culture media, but yields, desiccation tolerance, and storage stability varied with C:N ratio and carbon rate. Air-dried MS granules (<4 % moisture) retained excellent shelf life under cool and unrefrigerated storage conditions with no loss in conidial production. A low-cost complex nitrogen source based on cottonseed flour effectively supported high MS yields. Amending potting mix with dried MS formulations reduced or eliminated damping-off of melon seedlings caused by Rhizoctonia solani. Together, the results provide insights into the liquid culture production, stabilization process, and bioefficacy of the hitherto unreported MS of T. harzianum as a potential biofungicide for use in integrated management programs against soilborne diseases.
dc.identifierFungal Biology, v. 119, n. 4, p. 179-190, Apr. 2015.
dc.identifier1878-6146
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1011851
dc.identifier10.1016/j.funbio.2014.12.005
dc.identifier.urihttp://hdl.handle.net/123456789/396301
dc.languageeng
dc.rightsopenAccess
dc.subjectRhizoctonia solani
dc.subjectTrichoderma harzianum
dc.subjectBiopesticides
dc.subjectSoil-borne diseases
dc.subjectSclerotia
dc.subjectCarbon nitrogen ratio
dc.titleLiquid culture production of microsclerotia and submerged conidia by Trichoderma harzianum active against damping-off disease caused by Rhizoctonia solani.
dc.typeArtigo de periódico

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