Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

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dc.creatorMasake, R.A.
dc.creatorOle-MoiYoi, O.K.
dc.creatorUrakawa, T.
dc.creatorHirumi, H.
dc.creatorMajiwa, Phelix A.O.
dc.creatorWells, C.W.
dc.creatorMinja, S.H.
dc.creatorMakau, J.M.
dc.creatorNantulya, V.M.
dc.date1995-12
dc.date2013-05-06T07:00:59Z
dc.date2013-05-06T07:00:59Z
dc.date.accessioned2026-06-27T16:41:38Z
dc.descriptionA monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout the cytosol and nucleus of metacyclic forms, bloodstream forms, and procyclic-like elongated trypomastigotes, but is not detectable in epimastigotes of T. vivax. The T. vivax-specific antigen prepared from parasite lysates appeared to be of lower molecular mass than the form expressed in either Escherichia coli or in baculovirus-infected silkworm insect cells. In the recombinant baculovirus-infected cells, the protein was expressed mostly as an 18-kDa peptide with less abundant forms of 13 and 12 kDa, while the protein expressed in E. coli was approximately 14 kDa. Both the low- and higher-molecular-weight proteins are recognized by the MAb Tv27 in Western blots and in Ag-ELISA. Although the crude preparations of the protein produced by the insect cells are labile when kept for more than 2 hr at 24°C, they retained reactivity at temperatures below 4°C for several weeks. The proteins expressed in both the insect cells and E. coli captured anti-T. vivax antibodies in sera prepared from trypanosome-infected animals. Since the recombinant protein expressed in the baculovirus-infected cells is available in large homogenous quantities, it would serve as a positive control in Ag-ELISA and is also usable for antibody detection assays.
dc.identifierhttps://hdl.handle.net/10568/28608
dc.identifier.urihttp://hdl.handle.net/123456789/130617
dc.languageen
dc.publisherElsevier
dc.rightsLimited Access
dc.sourceExperimental Parasitology;81: 536-545
dc.subjecttrypanosomiasis
dc.subjecttrypanosoma vivax
dc.subjectescherichia coli
dc.subjectimmunology
dc.subjectdisease control
dc.subjectglossinidae
dc.subjectantibodies
dc.subjectanimal diseases
dc.subjectantigens
dc.subjectmonoclonal antibodies
dc.titleImmunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals
dc.typeJournal Article

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