Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype influence, medium optimization and compounds identification.

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dc.contributorRENATA LÚCIA GRUNENNVALDT, UFPR
dc.contributorJULIANA DEGENHARDT, CNPF
dc.contributorPETER BROOKS, University of The Sunshine Coast
dc.contributorJÉSSICA DE CÁSSIA TOMASI, UFPR
dc.contributorFABRICIO AUGUSTO HANSEL, CNPF
dc.contributorTRONG TRAN, University of The Sunshine Coast
dc.contributorERIK N. GOMES, The State University of New Jersey
dc.contributorCÍCERO DESCHAMPS, UFPR.
dc.creatorGRUNENNVALDT, R. L.
dc.creatorDEGENHARDT, J.
dc.creatorBROOKS, P.
dc.creatorTOMASI, J. de C.
dc.creatorHANSEL, F. A.
dc.creatorTRAN, T.
dc.creatorGOMES, E. N.
dc.creatorDESCHAMPS, C.
dc.date2020-11-12T19:28:38Z
dc.date2020-11-12T19:28:38Z
dc.date2020-11-12
dc.date2020
dc.date.accessioned2026-07-07T03:30:27Z
dc.descriptionIlex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine.
dc.identifierAnais da Academia Brasileira de Ciências , v. 92, n. 3, e20181251, 2020. 16 p.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1126527
dc.identifierhttps://doi.org/10.1590/0001-3765202020181251
dc.identifier.urihttp://hdl.handle.net/123456789/435781
dc.languageeng
dc.rightsopenAccess
dc.subjectBiomass accumulation
dc.subjectPlant growth regulators
dc.subjectErva mate
dc.subjectAtividade antioxidante
dc.subjectAcúmulo de biomassa
dc.subjectIlex Paraguariensis
dc.subjectSacarose
dc.subjectRegulador de Crescimento
dc.subjectYerba mate
dc.subjectSucrose
dc.subjectAntioxidant activity
dc.titleCallus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype influence, medium optimization and compounds identification.
dc.typeArtigo de periódico

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