Biochemical changes during plantlet regeneration in two accessions of Mucuna pruriens
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Society for Promotion of Horticulture
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The genus Mucuna is an important medicinal herb and is extensively used in traditional Indian systems of medicinefor various ailments. In vitro culture technique provides an alternative to plant propagation and germplasm conservation.Our aim was to study the biochemical changes occurring during regeneration of shoots (plantlets) from explants oftwo accessions of Mucuna pruriens, by monitoring the efficiency of nitrogen utilization and changes in levels of somehydrolytic enzymes. A rapid micropropagation system was developed using Murashige and Skoog’s (MS) mediumsupplemented with BAP and IAA combined. In both the accessions, 3.0mg l-1 6-BAP, in combination with 0.2mg l-1 IAA,induced shoot buds and shoot elongation; however for multiple-shoot induction, a slightly higher concentration ofcytokinin, i.e., 3.5mg l-1 6-BAP, in combination with 0.2mg l-1 IAA, was required. Results of the present study confirman active growth of explants revealed by nitrate assimilation enzymes and hydrolytic enzymes. It is concluded thatmedium composition, growth regulator combination and culture incubation conditions are all vital in both the accessionsof Mucuna pruriens for induction of in vitro plant regeneration.
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