African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro

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American Association for the Advancement of Science

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Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37°C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces.

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trypanosoma brucei, animal diseases

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http://hdl.handle.net/123456789/140399

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https://hdl.handle.net/10568/29601

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Instituto Internacional de Investigaciones Pecuarias (ILRI)