Characterization and functional validation of a genomic region involved in resistance to rust race II in Coffea arábica.

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dc.contributorCAROLINE ARIYOSHI, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; SUZANA TIEMI IVAMOTO-SUZUKI, UNIVERSIDADE ESTADUAL DE LONDRINA; VIVIANE YUMI BABA, UNIVERSIDADE ESTADUAL DE LONDRINA; MASAKO TOMA-BRAGHINI, INSTITUTO AGRONÔMICO; GUSTAVO HIROSHI SERA, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; ADRIAN POWELL, CORNELL UNIVERSITY; SANDRA MARIA BELLODI CAÇÃO, TROPICAL MELHORAMENTO & GENÉTICA; EVELINE TEIXEIRA CAIXETA MOURA, CNPCA; LUIZ FILIPE PROTASIO PEREIRA, CNPCA.
dc.creatorARIYOSHI, C.
dc.creatorIVAMOTO-SUZUKI, S. T.
dc.creatorBABA, V. Y.
dc.creatorTOMA-BRAGHINI, M.
dc.creatorSERA, G. H.
dc.creatorPOWELL, A.
dc.creatorCAÇÃO, S. M. B.
dc.creatorCAIXETA, E. T.
dc.creatorPEREIRA, L. F. P.
dc.date2025-05-28T05:46:49Z
dc.date2025-05-28T05:46:49Z
dc.date2024-08-30
dc.date2024
dc.date.accessioned2026-07-01T00:27:32Z
dc.descriptionCoffee leaf rust (CLR) is one of the most economically important diseases affecting Coffea arabica production, having a significant economic impact. Among the main goals of coffee breeding programs is the development of cultivars resistant to this disease. A source of resistance genes is Híbrido de Timor (HdT), a spontaneous hybrid originated from the cross between C. arabica and C. canephora. Previously, in a transcriptome study, the Ca TDF77 NBS-LRR gene from HdT involved in resistance to CLR was identified. Hence, our aim was to characterize the genomic region surrounding the Ca TDF77 NBS-LRR gene in Coffea spp. Furthermore, we aimed to analyze the transcriptional profile of this gene, in the C. arabica cultivar IAPAR 59, which is originated from HdT introgression and is resistant to CLR race II. The outcome delineated the gene’s localization on chromosome 11 (canephora subgenome) of C. arabica, spotlighting intragenic polymorphisms between HdT and Arabica coffee susceptible to CLR race II. The genomic region surrounding the gene in Coffea spp. revealed a tandem structure and transposable elements. Notably, within IAPAR 59, the gene exhibited significant upregulation at 24 and 72 h post CLR infection, contrasting starkly with the susceptible genotype. This observation validates its role in fortifying the defense mechanism of this particular cultivar. This study enriches our understanding of the evolutionary dynamics of Coffea spp. genomes and also provides genomic resources instrumental in devising biotechnological strategies for resistance to CLR.
dc.format11 p.
dc.identifierAustralasian Plant Pathology, 2024.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1166968
dc.identifier.urihttp://hdl.handle.net/123456789/404543
dc.languageeng
dc.rightsopenAccess
dc.subjectCoffea Arábica
dc.subjectLeaf rust
dc.subjectPlant breeding
dc.subjectGenetic resistance
dc.subjectTimor
dc.titleCharacterization and functional validation of a genomic region involved in resistance to rust race II in Coffea arábica.
dc.typeArtigo de periódico

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