In-Vitro Cytotoxicity of Aflatoxin B1 to Broiler Lymphocytes of Broiler Chickens

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dc.creatorCEP Zimmermann
dc.creatorAK Machado
dc.creatorFC Cadoná
dc.creatorJAS Jaques
dc.creatorKB Schlemmer
dc.creatorC Lautert
dc.creatorIBM Cruz
dc.creatorRA Zanette
dc.creatorDBR Leal
dc.creatorJM Santurio
dc.date2014
dc.date.accessioned2026-07-07T03:22:44Z
dc.descriptionThe aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 μg/mL AFB1 at 48 h (p < 0.05) and of 10 and 20 μg/mL AFB1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 μg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 μg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes.
dc.formatapplication/pdf
dc.identifier1516-635X
dc.identifierhttps://www.redalyc.org/articulo.oa?id=179732209012
dc.identifier.urihttp://hdl.handle.net/123456789/431994
dc.languageen
dc.publisherFundação APINCO de Ciência e Tecnologia Avícolas
dc.relationhttp://www.redalyc.org/revista.oa?id=1797
dc.rightsRevista Brasileira de Ciência Avícola
dc.sourceRevista Brasileira de Ciência Avícola (Brasil) Num.3 Vol.16
dc.subjectAgrociencias
dc.titleIn-Vitro Cytotoxicity of Aflatoxin B1 to Broiler Lymphocytes of Broiler Chickens
dc.typeartículo científico

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Revista Brasileira de Ciência Avícola - Fundação APINCO de Ciência e Tecnologia Avícolas