Analysis of gene expression of in vitro produced bovine blastocyst cultured for 72 hours post-vitrification.
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In vitro produced (IVP) embryos frequently show low survival rates after cryopreservation. Among the available methods, vitrification has generally shown better results with IVP embryos. However, little is known about vitrification effects on genes expression. The aim of this study was to evaluate the expression of aquaporins encoding genes for (AQP) 3, AQP11 and ATPase α1 in vitrified/warming embryos cultured in vitro for 72h. AQPs are channels that facilitate water transportation across membranes, whereas ATPase α1 is a Na/K pump subunit. Both AQPs and Na/K pump may be involved in blastocoel expansion after cryopreservation
Edição dos abstracts do 16º International Congress on Animal Reproduction, Budapest, 2008.
Edição dos abstracts do 16º International Congress on Animal Reproduction, Budapest, 2008.
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Expressão genética, Bovino