Efficiency of coffee seeds rna extraction protocol using RNA integrity number analysis.

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dc.contributorThe Ohio State University; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; The Ohio State University; LILIAN PADILHA, CNPCa.
dc.creatorSANTOS, F. C.
dc.creatorROSA, S. D. V. F. da
dc.creatorGROTEWOLD, E.
dc.creatorPADILHA, L.
dc.date2020-11-19T09:15:20Z
dc.date2020-11-19T09:15:20Z
dc.date2020-11-18
dc.date2014
dc.date.accessioned2026-07-01T00:26:57Z
dc.descriptionCoffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation.
dc.identifierIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 273-274.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1126783
dc.identifier.urihttp://hdl.handle.net/123456789/404298
dc.languagepor
dc.rightsopenAccess
dc.subjectExtração
dc.subjectRNA
dc.subjectSemente
dc.subjectCafé
dc.titleEfficiency of coffee seeds rna extraction protocol using RNA integrity number analysis.
dc.typeResumo em anais e proceedings

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