Two-dimensional gel electrophoresis to identify arcelins from Phaseolus vulgaris with inmuno-proteomic analysis

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dc.creatorCarolina Bernal
dc.creatorDaynet Sosa
dc.creatorIván Galindo-Castro
dc.creatorNardy Diez
dc.date2018
dc.date.accessioned2026-07-07T02:43:30Z
dc.descriptionArcelins are lectin-like proteins present in the common bean Phaseolus vulgaris that are believed to play a role in bruchid resistance. The present study used proteomics to analyze the expression of lectin-like proteins, specifically arcelins, in P. vulgaris cultivar varieties from Venezuela. A PAGE-SDS analysis of 30 commercial accessions of P. vulgaris showed significant differences in the molecular weight range of lectin-like proteins (arcelins). Eight different accessions were selected based on their electroforetic mobility for the proteomic analysis. Arcelin immuno-detection of two dimentional electrophoresed proteins was used to easily display the different arcelin proteomic profiles of the studied accessions. Mass spectrometry analysis confirmed the arcelin nature of these proteins. This is the first report on arcelin evaluation of the Venezuelan germoplasm of P. vulgaris with the aim of enhancing breeding programs by identifying accession materials with resistance to bean storage pests.
dc.formatapplication/pdf
dc.identifier0120-9965
dc.identifierhttps://www.redalyc.org/articulo.oa?id=180358913002
dc.identifierhttps://www.redalyc.org/journal/1803/180358913002/
dc.identifierhttps://www.redalyc.org/journal/1803/180358913002/html/
dc.identifierhttps://www.redalyc.org/journal/1803/180358913002/180358913002.epub
dc.identifierhttps://www.redalyc.org/journal/1803/180358913002/movil
dc.identifier10.15446/agron.colomb.v36n2.67694
dc.identifier.urihttp://hdl.handle.net/123456789/421927
dc.languageen
dc.publisherUniversidad Nacional de Colombia
dc.relationhttp://www.redalyc.org/revista.oa?id=1803
dc.rightsAgronomía Colombiana
dc.sourceAgronomía Colombiana (Colombia) Num.2 Vol.36
dc.subjectAgrociencias
dc.titleTwo-dimensional gel electrophoresis to identify arcelins from Phaseolus vulgaris with inmuno-proteomic analysis
dc.typeartículo científico

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Agronomía Colombiana - Universidad Nacional de Colombia