Enteric Viruses in Turkey Flocks: A Historic Review
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Fundação APINCO de Ciência e Tecnologia Avícolas
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In this review, diagnostic techniques and viral agents involved in enteric diseases affecting turkeys are described. Data from field observations and laboratory researches have been reported in turkey flocks for over 70 years, and several viruses have been identified. After a period of 30 years of inoculation experiments and neutralization studies, adequate visualization of the viruses was achieved using electronic microscopy. During the following years, several studies were then conducted to isolate and classify those viruses using cell-culture, embryo-propagation, serological tests, genome electropherotyping by polyacrylamide gel electrophoresis of double-stranded RNA viruses, and recently, nucleic acid studies. Thus, since the 1990s, the nucleicacid technology has focused on genomic surveys and on the detection of specific segments of the genome of each virus using the polymerasechain reaction, resulting in several prevalence studies and phylogenetic analyses of different isolates and proper classification of the viruses.
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