Insecticidal potential of parahancornia amapa (Apocynaceae) against atta sexdens: isolation and characterization of bioactive compounds.

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dc.contributorANDERSON DO REGO PIRES, UNIVERSIDADE FEDERAL DE SÃO CARLOS; SEAN DOS SANTOS ARAUJO, UNIVERSIDADE FEDERAL DE SÃO CARLOS; GABRIELE FERNANDA DA SILVA, UNIVERSIDADE FEDERAL DE SÃO CARLOS; EDUARDA OLIVEIRA DOS SANTOS, UNIVERSIDADE FEDERAL DE SÃO CARLOS; LUIS GUILLERMO CUADRADO DURANGO, UNIVERSIDADE FEDERAL DE SÃO CARLOS; DORAI PERIOTTO ZANDONAI, UNIVERSIDADE FEDERAL DE SÃO CARLOS; ODAIR CORREA BUENO, UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO; JOAO OIANO NETO, CPPSE; MARIA FÁTIMA DAS GRAÇAS FERNANDES DA SILVA, UNIVERSIDADE FEDERAL DE SÃO CARLOS; JOÃO BATISTA FERNANDES, UNIVERSIDADE FEDERAL DE SÃO CARLOS.
dc.creatorPIRES, A. do R.
dc.creatorARAUJO, S. dos S.
dc.creatorSILVA, G. F. da
dc.creatorSANTOS, E. O. dos
dc.creatorDURANGO, L. G. C.
dc.creatorZANDONAI, D. P.
dc.creatorBUENO, O. C.
dc.creatorOIANO NETO, J.
dc.creatorSILVA, M. F. das G. F. da
dc.creatorFERNANDES, J. B.
dc.date2025-12-02T14:49:00Z
dc.date2025-12-02T14:49:00Z
dc.date2025-12-02
dc.date2025
dc.date.accessioned2026-07-07T05:34:51Z
dc.descriptionAbstract: Leaf-cutter ants of the genera Atta and Acromyrmex rank among the most destructive agricultural pests due to their aggressive herbivory. The widespread and indiscriminate use of synthetic pesticides to control these insects has led to severe environmental impacts, threatening ecosystem integrity and posing significant public health risks. In this context, Natural Products Chemistry emerges as a sustainable and promising approach, enabling the isolation of secondary metabolites with potential insecticidal activity. The Amazonian species Parahancornia amapa (Huber) Ducke is traditionally used as a natural insecticide, was investigated through exhaustive extraction, followed by chromatographic fractionation using silica gel (230–400 mesh), high-performance liquid chromatography (HPLC), LC coupled with mass spectrometry (LC-MS/MS), and nuclear magnetic resonance (NMR), enabled the isolation of previously unreported compounds in this species. Toxicity bioassays conducted with extracts and fractions from the twigs and leaves of P. amapa against medium-sized worker ants of Atta sexdens guided the isolation of bioactive metabolites. From the twig fraction, two novel alkaloids, a new pterocarpan derivative, and a previously unreported benzopyran were isolated. From the leaf fraction, three compounds were identified: an aryl-naphthalene lactone Justicidin B and two known flavonoids catechin and quercetin. The bioactive fractions from both twigs and leaf, from which these compounds were isolated, induced 100% mortality (p < 0.05) of A. sexdens worker ants within 12 and 14 days, respectively. Furthermore, the twig fraction exhibited inhibitory activity against A. sexdens acetylcholinesterase (AsAChE), with IC₅₀ values of 12.96 mg·L⁻¹ (± 1.02) for isoform A and 9.51 mg·L⁻¹ (± 0.35) for isoform B, suggesting a neurotoxic mechanism of action. These findings highlight P. amapa as a promising natural source of insecticidal compounds, reinforcing its potential as an eco-friendly alternative to conventional synthetic pesticides for the control of leaf-cutter ants.
dc.description10 BCNP, 36 RESEM
dc.identifierIn: BRAZILIAN CONFERENCE ON NATURAL PRODUCTS, 10.; MEETING ON MICROMOLECULAR EVOLUTION, SYSTEMATICS, AND ECOLOGY, 36., 2025, Belo Horizonte. Abstracts [...]. Belo Horizonte: Universidade Federal de Minas Gerais, 2025. 1293307.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1182175
dc.identifier.urihttp://hdl.handle.net/123456789/495670
dc.languageeng
dc.rightsopenAccess
dc.subjectComposto bioativo
dc.subjectInseticida
dc.subjectApocynaceae
dc.subjectIsolamento
dc.subjectInsecticides
dc.subjectAtta sexdens
dc.titleInsecticidal potential of parahancornia amapa (Apocynaceae) against atta sexdens: isolation and characterization of bioactive compounds.
dc.typeResumo em anais e proceedings

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