Optimizing solid-state fermentation of Trichoderma harzianum in packed-bed bioreactors using beer spent grains and wheat straw for the production of metabolites related to phosphorus availability.

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dc.contributorFEDERAL UNIVERSITY OF SAO CARLOS; UNIVERSITAT AUTONOMA DE BARCELONA, CERDANYOLA DEL VALLES, SPAIN; UNIVERSITAT AUTONOMA DE BARCELONA, CERDANYOLA DEL VALLES, SPAIN; UNIVERSITAT AUTONOMA DE BARCELONA, CERDANYOLA DEL VALLES, SPAIN; FEDERAL UNIVERSITY OF SAO CARLOS; CRISTIANE SANCHEZ FARINAS, CNPDIA; UNIVERSITAT AUTONOMA DE BARCELONA, CERDANYOLA DEL VALLES, SPAIN.
dc.creatorRODRIGUES, N. A.
dc.creatorFONT, J.
dc.creatorPEÑATE, E. M.
dc.creatorSALA, A.
dc.creatorCASCIATORI, F. P.
dc.creatorFARINAS, C. S.
dc.creatorARTOLA, A.
dc.date2026-06-01T20:48:44Z
dc.date2026-06-01T20:48:44Z
dc.date2026-06-01
dc.date2026
dc.date.accessioned2026-07-07T04:30:00Z
dc.descriptionPhosphorus (P) availability is a key limiting factor for plant growth, and microbial-based strategies represent a promising approach for developing sustainable agricultural inputs. In this context, this study investigates the cultivation of Trichoderma harzianum using beer spent grains and wheat straw as substrates in a solid-state fermentation (SSF) process in packed bed bioreactors (PBBs) at lab and bench scales. The objective was to evaluate the strain’s potential to enhance P availability by promoting medium acidification and the production of phosphatases, indole derivatives, and flavonoids. The optimized process in 0.5 L PBB resulted in fermented solids with high spore content (1.08 ×1010 spores g 1 of dry mass, DM), acid phosphatase (ACP) activity (3.7 U g 1 DM), and phosphate mobilization. Additionally, the strain produced indole derivatives (511.3 µg g 1 DM) and total flavonoids (2.2 mg g 1 DM). Air flow and substrate ratio were the most significant factors, highlighting the role of adequate O2 supply and efficient CO2 removal in SSF processes. At bench scale (22 L PBB), cultivation also supported high biomass formation, at the order of 1 ×109 spores g 1 DM, and yielded appreciable amounts of indole derivatives (688 and 498 µg g DM in batches 1 and 2, respectively). Production of ACP and flavonoids was lower compared with lab scale, likely influenced by a 16.5 ◦ C temperature rise above the optimal range, underscoring the need to improve heat dissipation. These findings demonstrate the potential of PBBs for pro ducing multifunctional T. harzianum-based products from agro-industrial residues under solid-state fermentation.
dc.format15 p.
dc.identifierBioresource Technology, v. 456, 134917, 2026.
dc.identifier0960-8524
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1187296
dc.identifierhttps://doi.org/10.1016/j.biortech.2026.134917
dc.identifier.urihttp://hdl.handle.net/123456789/463257
dc.languageeng
dc.rightsopenAccess
dc.subjectPhosphate-solubilizing microorganisms
dc.subjectAgro-industrial residues
dc.subjectFungal cultivation
dc.subjectBiostimulants
dc.subjectMultifunctional bio-inputs
dc.subjectBioprocess scale-up
dc.titleOptimizing solid-state fermentation of Trichoderma harzianum in packed-bed bioreactors using beer spent grains and wheat straw for the production of metabolites related to phosphorus availability.
dc.typeArtigo de periódico

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