First detection of Theileria parva in cattle from Cameroon in the absence of the main tick vector Rhipicephalus appendiculatus

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dc.creatorSilatsa, Barberine A.
dc.creatorSimo, G.
dc.creatorGithaka, Naftaly W.
dc.creatorKamga, R.
dc.creatorOumarou, F.
dc.creatorTiambo, Christian K.
dc.creatorMachuka, Eunice M.
dc.creatorDomelevo Entfellner, Jean-Baka
dc.creatorOdongo, David O.
dc.creatorBishop, Richard P.
dc.creatorKuiate, Jules-Roger
dc.creatorNjiokou, F.
dc.creatorDjikeng, Appolinaire
dc.creatorPelle, Roger
dc.date2020-03
dc.date2020-04-11T17:50:16Z
dc.date2020-04-11T17:50:16Z
dc.date.accessioned2026-06-27T16:59:15Z
dc.descriptionA major risk factor for the spread of livestock diseases and their vectors is the uncontrolled transboundary movement of live animals for trade and grazing. Such movements constrain effective control of tick-transmitted pathogens, including Theileria parva. Only limited studies have been undertaken to identify ticks and tick-borne diseases (TTBDs) affecting cattle in central African countries, including Cameroon. We hereby report the collection of baseline data on the prevalence of T. parva in Cameroon through a countrywide cross-sectional survey, conducted in 2016, involving collection of blood samples from cattle from 63 sites across the five agro-ecological zones (AEZs) of the country. ELISA-based surveillance of infected cattle was performed on 479 randomly selected samples and revealed specific antibodies to T. parva in 22.7% and T. mutans in 41.1% of cattle. Screening of 1,340 representative DNA samples for the presence of T. parva identified 25 (1.86%) positives using a p104 antigen gene-based nested PCR assay. The positives were distributed across agro-ecological zones I, II, III and V. None of the p104 positive cattle exhibited clinical symptoms of East Coast fever (ECF). Using reverse line blot (RLB), 58 (4.3%) and 1,139 (85%) of the samples reacted with the T. parva and T. mutans oligonucleotide probes, respectively. This represents the first report of T. parva from Cameroon. Surprisingly, no Rhipicephalus appendiculatus ticks, the main vector of T. parva, were identified in a parallel study involving comprehensive morphological and molecular survey of tick species present in the country. Only two of the 25 p104 positive cattle were PCR-positive for the CD8+ T-cell target schizont-expressed antigen gene Tp1. Cloning and sequencing of Tp1 amplicons revealed sequence identity with the reference T. parva Muguga. This new finding raises serious concerns of a potential spread of ECF into the central African region.
dc.identifierhttps://hdl.handle.net/10568/107968
dc.identifier.urihttp://hdl.handle.net/123456789/138898
dc.languageen
dc.publisherHindawi Limited
dc.rightsOpen Access
dc.sourceSilatsa, B.A., Simo, G., Githaka, N., Kamga, R., Oumarou, F., Tiambo, C.K., Machuka, E., Domelevo, J.-B., Odongo, D., Bishop, R., Kuiate, J.-R., Njiokou, F., Djikeng, A. and Pelle, R. 2020. First detection of Theileria parva in cattle from Cameroon in the absence of the main tick vector Rhipicephalus appendiculatus. Transboundary and Emerging Diseases 67(Suppl. 1):68–78.
dc.subjectanimal diseases
dc.subjectdisease control
dc.subjectcattle
dc.subjectlivestock
dc.titleFirst detection of Theileria parva in cattle from Cameroon in the absence of the main tick vector Rhipicephalus appendiculatus
dc.typeJournal Article

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