Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin

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dc.creatorNarayanan, Narayanan
dc.creatorBeyene, Getu
dc.creatorChauhan, Raj Deepika
dc.creatorGaitán-Solís, Eliana
dc.creatorGehan, Jackson
dc.creatorBoy, Erick
dc.date2019-02-07
dc.date2024-06-21T09:06:50Z
dc.date2024-06-21T09:06:50Z
dc.date.accessioned2026-06-27T15:21:36Z
dc.descriptionLess than 10% of the estimated average requirement (EAR) for iron and zinc is provided by consumption of storage roots of the staple crop cassava (Manihot esculenta Crantz) in West African human populations. We used genetic engineering to improve mineral micronutrient concentrations in cassava. Overexpression of the Arabidopsis thaliana vacuolar iron transporter VIT1 in cassava accumulated three- to seven-times-higher levels of iron in transgenic storage roots than nontransgenic controls in confined field trials in Puerto Rico. Plants engineered to coexpress a mutated A. thaliana iron transporter (IRT1) and A. thaliana ferritin (FER1) accumulated iron levels 7–18 times higher and zinc levels 3–10 times higher than those in nontransgenic controls in the field. Growth parameters and storage-root yields were unaffected by transgenic fortification in our field data. Measures of retention and bioaccessibility of iron and zinc in processed transgenic cassava indicated that IRT1 + FER1 plants could provide 40–50% of the EAR for iron and 60–70% of the EAR for zinc in 1- to 6-year-old children and nonlactating, nonpregnant West African women.
dc.identifierhttps://hdl.handle.net/10568/146376
dc.identifier.urihttp://hdl.handle.net/123456789/102094
dc.languageen
dc.publisherNature Publishing Group
dc.rightsOpen Access
dc.sourceNarayanan, Narayanan; Beyene, Getu; Chauhan, Raj Deepika; Gaitán-Solís, Eliana; Gehan, Jackson; Boy, Erick; et al. 2019. Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin. Nature Biotechnology 37(2): 144–151. https://doi.org/10.1038/s41587-018-0002-1
dc.subjectbiofortification
dc.subjectgenetic engineering
dc.subjectcassava
dc.subjectiron
dc.subjectzinc
dc.titleBiofortification of field-grown cassava by engineering expression of an iron transporter and ferritin
dc.typeJournal Article

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