Improvement of recovery cycle of cryopreserved potato shoot tips – Small changes, but huge impact

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dc.creatorVollmer, R.
dc.creatorEspirilla, J.
dc.creatorVillagaray, R.
dc.creatorCastro, M.
dc.creatorCardenas, J.
dc.creatorPineda, S.
dc.creatorAzevedo, V.C.R.
dc.date2022-12
dc.date2023-03-23T16:46:48Z
dc.date2023-03-23T16:46:48Z
dc.date.accessioned2026-06-27T17:58:08Z
dc.descriptionImprovement and optimization of cryopreservation protocols for its reliable large-scale application is a time-consuming process. The development of robust and efficient protocols implicates the assessment of a high number of diverse genotypes before results can be extrapolated on complete collections. To simplify and improve the recovery cycle of potato shoot tips cryopreserved with the droplet-vitrification method, it was assessed if placing shoot tips after thawing for 9 days on culture medium with normal and stable sucrose concentration of 0.073 M (in darkness), can increase the viability rate, compared to a stepwise decrease of the sucrose concentration from 0.3 M to 0.2 M to 0.1M and 0.073 M during the initial phase of the recovery cycle (control treatment). The experiment was performed with 68 diverse potato landraces which showed a significantly higher recovery rate of 69.8 % when shoot tips were recovered on culture medium with a stable sucrose concentration of 0.073 M, compared to the control treatment (57.9 %). The difference in recovery rates was confirmed during routine cryopreservation: a set of 806 accessions recovered with the control treatment showed an average recovery rate of 60.9 % (years 2018-19), while 1158 accessions processed with the new protocol had an average recovery rate of 75.1 % (2019-now). Currently, CIP conserves 4017 virus-free potato accessions in its cryobank, which represent about 83% of the in vitro collection (4851 accessions). Based on a high experimental sample size of 68 tested accessions (1.4 % of population) robust and reliable results about the cryo-response of the whole collection were obtained.
dc.identifierhttps://hdl.handle.net/10568/129750
dc.identifier.urihttp://hdl.handle.net/123456789/150964
dc.languageen
dc.publisherElsevier
dc.rightsLimited Access
dc.sourceVollmer, R.; Espirilla, J.; Villagaray, R.; Castro, M.; Cárdenas, J.; Pineda, S.; Azevedo, V. 2022. Improvement of recovery cycle of cryopreserved potato shoot tips – Small changes, but huge impact. Cryobiology, 109, 13–14. https://doi.org/10.1016/j.cryobiol.2022.11.044
dc.subjectcryopreservation
dc.subjectpotatoes
dc.titleImprovement of recovery cycle of cryopreserved potato shoot tips – Small changes, but huge impact
dc.typeJournal Article

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