Increased iNOS gene expression in the granulosa layer of F1 follicle of over-fed and under-fed broiler breeder hens
| dc.creator | A Sheikh Ahmadi | |
| dc.creator | M Zaghari | |
| dc.creator | M Shivazad | |
| dc.creator | H Hassanpour | |
| dc.creator | A Towhidi | |
| dc.date | 2010 | |
| dc.date.accessioned | 2026-07-07T03:23:53Z | |
| dc.description | To clarify the effects of high (20 and 40% more than normal) and low (20% less than normal) daily feed allowance on egg and body parameters, ovarian morphology and plasma glucose, cholesterol, triacylglycerol, leptin-like hormone, nitrite/nitrate and gene expression of inducible nitric oxide synthase (iNOS) in the granulosa layer of F1 follicle, broiler breeder hens (30-week-old) were fed for 30 days. Egg and body parameters significantly changed between treatments (p<0.05). Effect of different level of feed intake on ovarian morphology parameters was significant (p<0.05), except for white follicles. After 30 days of experiment, plasma nitrite/nitrate (as a index of plasma nitric oxide) and leptin-like hormone increased in FI+20% and FI+40% groups as compared to controls (p<0.05). Plasma level of leptin-like hormone also significantly (p<0.05) increased in FI+40% group. The relative amount of iNOS mRNA expression in the granulosa layer of F1 follicles was significantly higher in FI-20% and FI+40% groups than in control group only after 4 weeks of experiment. The amount of these elevations in the FI-20% and FI+40% groups were 32.4% and 60.9% respectively. It was concluded that iNOS gene is normally expressed in follicular granulosa cells of F1 follicle of broiler breeder hens 2-4 hours before ovulation. However, over- and underfeeding of hens increased iNOS expression in F1 follicle, which may be one of the atresia-inducing factors in hierarchical follicles as shown by the significant (p<0.05) increase of shrunken follicles in the ovary of over- and under-fed broiler breeder hens after 30 days of feeding. | |
| dc.format | application/pdf | |
| dc.identifier | 1516-635X | |
| dc.identifier | https://www.redalyc.org/articulo.oa?id=179715864004 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/432724 | |
| dc.language | en | |
| dc.publisher | Fundação APINCO de Ciência e Tecnologia Avícolas | |
| dc.relation | http://www.redalyc.org/revista.oa?id=1797 | |
| dc.rights | Revista Brasileira de Ciência Avícola | |
| dc.source | Revista Brasileira de Ciência Avícola (Brasil) Num.4 Vol.12 | |
| dc.subject | Agrociencias | |
| dc.title | Increased iNOS gene expression in the granulosa layer of F1 follicle of over-fed and under-fed broiler breeder hens | |
| dc.type | artículo científico |