Molecular Characterization of a Spontaneous Waxy Starch Mutation in Cassava

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dc.creatorAiemnaka, Pornsak
dc.creatorWongkaew, Arunee
dc.creatorChanthaworn, Jumnong
dc.creatorNagashima,Sathakupt Ken
dc.creatorBoonma, Supawadee
dc.creatorAuthapun, Jetsada
dc.creatorJenweerawat, Sujin
dc.creatorKongsila, Pasajee
dc.creatorKittipadakul, Piya
dc.creatorNakasathien, Sutkhet
dc.creatorSreewongchai, Tanee
dc.creatorWannarat, Wannasiri
dc.creatorVichukit, Vichan
dc.creatorBecerra López Lavelle, Luis Augusto
dc.creatorCeballos, Hernán
dc.creatorRojanaridpiched ,Chareinsuk
dc.creatorPhumichai, Chalermpol
dc.date2012-09
dc.date2014-11-12T13:42:15Z
dc.date2014-11-12T13:42:15Z
dc.date.accessioned2026-06-27T14:39:37Z
dc.descriptionCassava (Manihot esculenta Crantz) starch is traded in international markets more than starch from any other source. The starch industry requires cassava cultivars with novel starch characteristics for commercial exploitation. A natural source of waxy (amylose‐free) cassava starch, clone AM 206‐5, was identified at the International Center for Tropical Agriculture (CIAT). The granule‐bound starch synthase I (GBSSI) gene is related to the waxy starch trait. The objectives of this study were to introgress the waxy starch mutation into Thailand's cassava breeding populations, to analyze the inheritance of waxy starch in cassava, and to develop molecular markers for this trait. AM 206‐5 (wxwx) was crossed with several elite cultivars (WxWx) to transfer the wx allele. Unrelated “F1” (Wxwx) genotypes were crossed among themselves to produce a “F2” segregating population with negligible levels of inbreeding depression. The waxy starch phenotype was recovered in 25.7% of the 11,192 genotypes tested. This result provides further evidence that cassava is a functional diploid and that only one copy of GBSSI is present in cassava. A full‐length genomic DNA sequence of GBSSI was isolated and characterized from C8, a waxy starch “F2” clone derived from AM 206‐5. We exploited these sequence differences to develop two diagnostic single‐nucleotide‐amplified polymorphism (SNAP) markers to differentiate homozygous waxy (wxwx) from the heterozygous (Wxwx) and homozygous (WxWx) nonwaxy genotypes.
dc.identifierhttps://hdl.handle.net/10568/51403
dc.identifier.urihttp://hdl.handle.net/123456789/84701
dc.languageen
dc.publisherWiley
dc.rightsLimited Access
dc.sourceAiemnaka, Pornsak; Wongkaew, Arunee; Chanthaworn, Jumnong; Nagashima, Sathakupt Ken; Boonma, Supawadee; Authapun, Jetsada; Jenweerawat, Sujin; Kongsila, Pasajee; Kittipadakul, Piya; Nakasathien, Sutkhet; Sreewongchai, Tanee; Wannarat, Wannasiri; Vichukit, Vichan; Becerra López-Lavalle, Luís Augusto; Ceballos, Hernán. 2012. Molecular Characterization of a Spontaneous Waxy Starch Mutation in Cassava. Crop Science. 52: 2121-2130.
dc.subjectmanihot esculenta
dc.subjectgenotypes
dc.subjectclones
dc.subjectstarch
dc.subjectintrogression
dc.subjectmutation
dc.subjectgenetic markers
dc.subjectpolymorphism
dc.subjectgenotipos
dc.subjectalmidón
dc.subjectintrogresión
dc.subjectmutación
dc.subjectmarcadores genéticos
dc.subjectpolimorfismo
dc.titleMolecular Characterization of a Spontaneous Waxy Starch Mutation in Cassava
dc.typeJournal Article

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