Enabling genome editing in tropical maize lines through an improved, morphogenic regulator-assisted transformation protocol.

HERNANDES-LOPES, J.; PINTO, M. S.; VIEIRA, L. R.; MONTEIRO, P. B.; GERASIMOVA, S. V.; NONATO, J. V. A.; BRUNO, M. H. F.; VIKHOREV, A.; FERNANDES, F. R.; GERHARDT, I. R.; PAUWELS, L.; ARRUDA, P.; DANTE, R. A.; YASSITEPE, J. E. de C. T.

Enabling genome editing in tropical maize lines through an improved, morphogenic regulator-assisted transformation protocol.

Autores

Descripción

Here, we report the successful GE of agronomically relevant tropical maize lines using a MR-based, Agrobacterium-mediated transformation protocol previously optimized for the B104 temperate inbred line.
Na publicação: Fernanda Rausch-Fernandes.

Palabras clave

Agrobactéria, Edição de genoma, B104, BABY BOOM, CRISPR/Cas9, Off-target, Protoplast, VIRESCENT YELLOW-LIKE, WUSCHEL, Protoplasto, Zea Mays, Milho, Agrobacterium