Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine.

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dc.contributorDANIELLE COSTENARO-DA-SILVA, UFRGS; GISELE PASSAIA, CNPUV (bolsista); JOÃO A. P. HENRIQUES, UFRGS; ROGÉRIO MARGIS, UFRGS; GIANCARLO PASQUALI, UFRGS; LUIS FERNANDO REVERS, CNPUV.
dc.creatorCOSTENARO-DA-SILVA, D.
dc.creatorPASSAIA, G.
dc.creatorHENRIQUES, J. A. P.
dc.creatorMARGIS, R.
dc.creatorPASQUALI, G.
dc.creatorREVERS, L. F.
dc.date2019-04-27T00:41:45Z
dc.date2019-04-27T00:41:45Z
dc.date2011-01-14
dc.date2010
dc.date2019-05-20T11:11:11Z
dc.date.accessioned2026-07-07T11:26:34Z
dc.descriptionSultanine grapevine (Vitis vinifera L.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. Despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. Actually, studies concerning berry development in seedless grapes are scarce at the molecular level. We therefore developed a representational difference analysis (RDA) modified method named Bulk Representational Analysis of Transcripts (BRAT) in the attempt to identify genes specifically associated with each of the main developmental stages of Sultanine grapevine berries. A total of 2400 transcript-derived fragments (TDFs) were identified and cloned by RDA according to three specific developmental berry stages. After sequencing and in silico analysis, 1554 (64.75%) TDFs were validated according to our sequence quality cut-off. The assembly of these expressed sequence tags (ESTs) yielded 504 singletons and 77 clusters, with an overall EST redundancy of approximately 67%. Amongst all stage-specific cDNAs, nine candidate genes were selected and, along with two reference genes, submitted to a deeper analysis of their temporal expression profiles by reverse transcription-quantitative PCR. Seven out of nine genes proved to be in agreement with the stage-specific expression that allowed their isolation by RDA.
dc.format510-519
dc.identifierPlant Science, Limerick, v. 179, n. 5, p. 510-519, nov. 2010.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/873059
dc.identifier.urihttp://hdl.handle.net/123456789/508015
dc.languageeng
dc.rightsopenAccess
dc.subjectUva sem semente
dc.subjectExpressão gênica
dc.subjectRDA
dc.subjectDesenvolvimento da baga
dc.subjectPCR em tempo real
dc.subjectUva de mesa
dc.subjectIdentificação genética
dc.subjectViticultura
dc.subjectUva
dc.subjectBiologia molecular
dc.subjectGenética
dc.titleIdentification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine.
dc.typeArtigo de periódico

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