Conformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays

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dc.creatorBarrera, Nubia F.
dc.creatorMelgarejo, Luz M.
dc.creatorCruz Gallego, Maribel
dc.creatorCortés, Lina Jimena
dc.creatorGuzmán, Fanny
dc.creatorCalvo, Julio C.
dc.date2018-09-05
dc.date2018-09-24T15:16:21Z
dc.date2018-09-24T15:16:21Z
dc.date.accessioned2026-06-27T14:28:44Z
dc.descriptionThe rice hoja blanca virus (RHBV), transmitted by the planthopper insect Tagosodes orizicolus, is a disease that attacks rice and generates significant production losses in Colombia. Fedearroz 2000 and Colombia I commercial rice varieties, which have different resistance levels to the disease, were selected in this study. To identify proteins associated to the insect and virus signaling, a comparative proteomics study was performed. By comparing proteomic profiles, between virus-infected and control group plants in two-dimensional electrophoresis, proteins exhibiting significant changes in abundance were found. In another test, peptide dendrimers containing sequences conformationally restricted to α-helix from four of those rice proteins were synthesized. In the experiment, sera from mice inoculated with peptide dendrimers could recognize the corresponding native protein in ELISA assays. Reported comparative proteomic results provide new insights into the molecular mechanisms of plant response to the RHBV and comprehensive tools for the analysis of new crop varieties. Besides, results from conformational peptide dendrimer approach are promising and show that it is feasible to detect proteins as markers, and may have biological applications by decreasing the susceptibility to proteolytic degradation.
dc.identifierhttps://hdl.handle.net/10568/97480
dc.identifier.urihttp://hdl.handle.net/123456789/79401
dc.languageen
dc.publisherMDPI
dc.rightsOpen Access
dc.sourceBarrera, N. F., Melgarejo, L. M., Cruz-Gallego, Maribel, Cortés, L. J., Guzmán, F., & Calvo, J. c. (2018). Conformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays. Molecules, 23(9), 2262.
dc.subjecttagosodes orizicolus
dc.subjectoryza sativa
dc.subjectpest insects
dc.subjectinsectos dañinos
dc.subjectpest resistance
dc.subjectresistencia a las plagas
dc.subjectviruses
dc.subjectanalytical chemistry
dc.titleConformationally Restricted Peptides from Rice Proteins Elicit Antibodies That Recognize the Corresponding Native Protein in ELISA Assays
dc.typeJournal Article

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