Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone

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dc.creatorWambugu, J.
dc.creatorKanguha, E.
dc.creatorHanotte, Olivier H.
dc.creatorDavis, S.
dc.creatorTaylor, J.
dc.creatorSkow, L.
dc.creatorBrunner, R.
dc.creatorSchwerin, M.
dc.creatorTeale, A.J.
dc.creatorIraqi, F.A.
dc.date2001
dc.date2013-05-06T07:00:15Z
dc.date2013-05-06T07:00:15Z
dc.date.accessioned2026-06-27T16:46:08Z
dc.descriptionTwo bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing sequences homologous with the probe was sequenced and a (GT)9 microsatellite was identified. Further DNA sequencing for the subclones yielded a (TA)10(TTA)5 microsatellite. Primers were designed to amplify these 2 microsatellites in 4 cattle breeds. The PCR product length was polymorphic as shown by denaturing polyacrylamide gel electrophoresis. The microsatellites were designated as ILFI-TNF-I and II for (GT)9 and (TA)10(TTA)5, respectively.
dc.identifierhttps://hdl.handle.net/10568/28253
dc.identifier.urihttp://hdl.handle.net/123456789/132643
dc.languageen
dc.rightsLimited Access
dc.sourceAnimal Genetics;32(2): 105-121
dc.subjectcattle
dc.subjectgenes
dc.subjectgenetic polymorphism
dc.subjectmicrosatellites
dc.subjectnucleotide sequences
dc.subjecttumour necrosis factor
dc.titleIdentification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)