Primer development for detection of Phaseolus vulgaris and Olathe transgenic bean.

DINON, A. Z.; BROD, F. C. A.; MELLO, C. S.; SILVEIRA, J. C. M.; KOLLING, D. J.; TREML, D.; FARIA, J. C.; ARISI, A. C. M.

Primer development for detection of Phaseolus vulgaris and Olathe transgenic bean.

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Descripción

Several strategies have been employed for genetically engineering resistance to viruses in transgenic plants. The bean golden mosaic virus (BGMV) is responsible for causing the gold mosaic of common bean (Phaseolus vulgaris L.) and causes severe yield losses due to yellow-green mosaic of leaves, stunted growth and distorted pods. Olathe transgenic bean (Olathe 5.1) was successfully produced by EMBRAPA using the RNA interference (RNAi) concept to silence the rep viral gene in common bean to generate transgenic lines with strong resistance to BGMV. In this work, real time PCR (RT-PCR) method with SYBR Green was developed to detect this newly genetically modified (GM) plant.