Cryopreservation of Coffea arabica L. zygotic embryos by vitrification.

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dc.contributorRODRIGO THEREZAN DE FREITAS, UFLA; RENATO PAIVA, UFLA; THAIS SILVA SALES, UFLA; DIOGO PEDROSA CORRÊA DA SILVA, UFLA; MICHELE VALQUÍRIA DOS REIS, UFLA; ANA CRISTINA DE SOUZA, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC.
dc.creatorFREITAS, R. T. da
dc.creatorPAIVA, R.
dc.creatorSALES, T. S.
dc.creatorSILVA, D. P. C. da
dc.creatorREIS, M. V. dos
dc.creatorSOUZA, A. C. de
dc.creatorROSA, S. D. V. F. da
dc.date2017-02-22T11:11:11Z
dc.date2017-02-22T11:11:11Z
dc.date2017-02-22
dc.date2016
dc.date2017-03-03T11:11:11Z
dc.date.accessioned2026-07-01T00:26:25Z
dc.descriptionAs a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.
dc.identifierNotulac Botanicae Horti Agrobotanici Cluj-Napoca, v. 44, n. 2, p. 445-451, Dec. 2016.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1065188
dc.identifier.urihttp://hdl.handle.net/123456789/404077
dc.languageeng
dc.rightsopenAccess
dc.subjectLiquid nitrogen
dc.subjectPVS2
dc.subjectTTC test
dc.subjectEx situ conservation
dc.subjectHistology
dc.subjectThawing
dc.titleCryopreservation of Coffea arabica L. zygotic embryos by vitrification.
dc.typeArtigo de periódico

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