Avaliação da toxicidade de solvente de extratos vegetais com ação antiviral em sêmen caprino refrigerado.

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dc.contributorRENATO MESQUITA PEIXOTO, UNIVERSIDADE ESTADUAL DO CEARÁ
dc.contributorALICE ANDRIOLI PINHEIRO, CNPC
dc.contributorDIONES OLIVEIRA SANTOS, CNPC
dc.contributorRAYMUNDO RIZALDO PINHEIRO, CNPC
dc.contributorJUSCILÂNIA FURTADO ARAÚJO, UNIVERSIDADE ESTADUAL DO CEARÁ
dc.contributorANA LÍDIA MADEIRA DE SOUSA, UNIVERSIDADE ESTADUAL DO CEARÁ
dc.contributorDAVI FARIA SILVA, INSTITUTO SUPERIOR DE TEOLOGIA APLICADA FACULDADES INTA
dc.contributorEDGAR MARQUES DAMASCENO, UNIVERSIDADE ESTADUAL VALE DO ACARAÚ
dc.contributorMARIA FÁTIMA DA SILVA TEIXEIRA, UNIVERSIDADE ESTADUAL DO CEARÁ.
dc.creatorPEIXOTO, R. M.
dc.creatorANDRIOLI, A.
dc.creatorSANTOS, D. O.
dc.creatorPINHEIRO, R. R.
dc.creatorARAÚJO, J. F.
dc.creatorSOUSA, A. L. M. de
dc.creatorSILVA, D. F.
dc.creatorDAMASCENO, E. M.
dc.creatorTEIXEIRA, M. F. da S.
dc.date2017-09-24T00:13:57Z
dc.date2017-09-24T00:13:57Z
dc.date2017-09-22
dc.date2017
dc.date2020-01-22T11:11:11Z
dc.date.accessioned2026-07-01T00:20:42Z
dc.description[Evaluation of Solvent Toxicity of Plant Extract with Antiviral Action in Refrigerated Goat Semen]. Background: Caprine Arthritis Encephalitis Virus have been detected in sperm of breeding goats causing economic losses. In order to control the virus, researches aiming to identify natural extracts with potential antiviral effects are performed. However, aqueous or ethanolic extracts must be diluted in dimethyl sulfoxide (DMSO), which is a substance with unknown effects in sperm quality when present in diluting media. Therefore, this study aimed to evaluate sperm viability of refrigerated caprine semen diluted in media containing DMSO. This was performed to provide data that aid in researches involving the use of this component with natural extracts that may inactivate the caprine lentivirus in sperm. Materials, Methods & Results: The experiment was performed at the Laboratory of Seminal Technology in Embrapa Goats and Sheep in the city of Sobral, Brazil. Sperm viability was assessed in caprine semen refrigerated in two dilution media with crescent concentrations of DMSO. Sperm samples of five goats seronegative for the caprine lentivirus were pooled and diluted in minimal essential medium (MEM) enriched with glucose at 0.01 M added of crescent concentrations of DMSO (0%, 1.5%, 1.75%, 2.0%, 2.25% and 2.5%). The same breeders provided the pool of sperm to test Tris added 2.5% of egg yolk and the same concentrations of DMSO previously mentioned. Treatments were refrigerated at 7°C and evaluated up until four h after DMSO addition. Individual progressive motility (MIP), sperm vigor (V), percentage of spermatozoa reactive to hypoosmotic test (HO) and morphologically normal (NOR) were evaluated. IPM, vigor and NOR remained within normal standards for the caprine species in all treatments test. Percentage results of spermatozoa reactive to hypoosmotic was higher in Tris yolk with values ranging between 34.66% to 46.33%. Sperm vigor was positively correlated (r = 0.85) with IPM in the MEM diluted pool of sperm. In Tris yolk, vigor and hypoosmotic test correlated moderately (r = 0.63, r = 0.54, respectively) with IPM. Tris yolk medium added DMSO presented the highest percentage of reactivity to hypoosmotic test in all treatments when compared to MEM added DMSO. Discussion: The fact that DMSO is easily homogenized in water, ethylic alcohol and most organic solvents favors its use in diluting natural extracts. These components are a possible source of products that inactivate caprine arthritis encephalitis virus in sperm, which is the key to promoting the safe use of genetic material of infected breeders, in addition to commercial use of germplasm. In this study, there was no interference of DMSO in the analyzed parameters when added in a maximum concentration of 2.5% to MEM and Tris yolk, which is in accordance with standard values for goats. In addition, Tris yolk may promote greater protection to the membrane of sperm cells, which was demonstrated by hypoosmotic test. This medium could be ideal to be used in new methodologies that incorporate DMSO. In conclusion, DMSO added to dilution media Tris yolk and MEM did not interfere with the quality of refrigerated caprine sperm, which maintained viability. These results indicate that this substance did not present harmful effects to the genetic material, promoting the use as solvent of extracts from plant compounds with potential anti-viral effect. The information in this study may aid new research performed in this area.
dc.descriptionTítulo em inglês: Evaluation of solvent toxicity of plant extract with antiviral action in refrigerated goat semen.
dc.identifierActa Scientiae Veterinariae, v. 45, n. 1487, p. 1-8, 2017.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1076169
dc.identifier.urihttp://hdl.handle.net/123456789/402258
dc.languagepor
dc.rightsopenAccess
dc.subjectTris yolk
dc.subjectTris Gema
dc.subjectMEM
dc.subjectDimetilsulfóxido
dc.subjectFitocomposto
dc.subjectDiluente
dc.subjectFitoterápico
dc.subjectPreservação do sêmen
dc.subjectAntimicrobial properties
dc.subjectCaprine arthritis encephalit virus
dc.subjectSemen diluents
dc.subjectSemen preservation
dc.subjectCaprino
dc.subjectSêmen
dc.subjectExtrato vegetal
dc.subjectCriopreservação
dc.subjectGoats
dc.subjectPlant extracts
dc.subjectBrazil
dc.subjectdimethyl sulfoxide
dc.titleAvaliação da toxicidade de solvente de extratos vegetais com ação antiviral em sêmen caprino refrigerado.
dc.typeArtigo de periódico

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