High-throughput sequencing in phytopathology: genomics-driven diagnostics and host-pathogen interactions.

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dc.contributorLAURA ROSSETTO PEREIRA, INSTITUTO BIOLÓGICO; MATHEUS POTSCLAM BARRO, INSTITUTO BIOLÓGICO, BRAZIL, UNIVERSIDADE DE SÃO PAULO, BRAZIL; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO; JULIANA DE FREITAS ASTUA, CNPMF; PEDRO LUIS RAMOS-GONZÁLEZ, INSTITUTO BIOLÓGICO.
dc.creatorPEREIRA, L. R.
dc.creatorBARRO, M. P.
dc.creatorHARAKAVA, R.
dc.creatorASTUA, J. de F.
dc.creatorRAMOS-GONZÁLEZ, P. L.
dc.date2026-05-27T11:49:07Z
dc.date2026-05-27T11:49:07Z
dc.date2026-05-27
dc.date2026
dc.date.accessioned2026-07-07T04:53:08Z
dc.descriptionABSTRACT: Plant diseases severely constrain agricultural productivity, exacerbating food insecurity, economic instability, and environmental degradation. Global trade and climate change further intensify pathogen spread, emergence, and host shifts. While traditional diagnostics and targeted assays, such as polymerase chain reaction and enzyme-linked immunosorbent assay, improve specificity, they depend on prior knowledge and are limited in detecting novel or mixed infections. High-throughput sequencing (HTS) has emerged as a transformative, unbiased platform that allows comprehensive detection of known and unknown pathogens through metagenomics and transcriptomics. By generating large-scale genomic data, HTS supports pathogen discovery, epidemiological surveillance, quarantine systems, and genome-informed disease management. It underpins advanced strategies, including Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) proteins editing and RNA interference, and accelerates the breeding of resistance. Despite challenges – such as bioinformatics standardization, cost, and data interpretation – HTS, when integrated with classical diagnostics and biological validation, represents a foundational technology for sustainable, proactive plant health management and global phytosanitary resilience.</jats:p>
dc.identifierArquivos do Instituto Biológico, v.93, n., p., 2026.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1187141
dc.identifierhttps://doi.org/10.1590/1808-1657000032026
dc.identifier.urihttp://hdl.handle.net/123456789/474213
dc.languageeng
dc.relation1808-1657
dc.rightsopenAccess
dc.subjectDoença de Planta
dc.subjectHospedeiro Vegetal
dc.subjectPatógeno
dc.subjectEpidemiologia
dc.subjectPlant diseases and disorders
dc.subjectHost-pathogen relationships
dc.subjectEpidemiology
dc.titleHigh-throughput sequencing in phytopathology: genomics-driven diagnostics and host-pathogen interactions.
dc.typeArtigo de periódico

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