Determination of moxidectin in serum by liquid chromatography-tandem mass spectrometry and its application in pharmacokinetic study in lambs.

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dc.contributorRAFAELA DE CARVALHO BAPTISTA, FEA-UNICAMP; MARIA ANGELA MACHADO FERNANDES, LAPOC-UFPR; SUSANA GILAVERTE, LAPOC-UFPR; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; MARCIA REGINA ASSALIN, CNPMA; VERA LUCIA FERRACINI, CNPMA; ALDA LUCIA GOMES MONTEIRO, LAPOC-UFPR; FELIX GUILLERMO REYES REYES, FEA-UNICAMP.
dc.creatorBAPTISTA, R. C.
dc.creatorFERNANDES, M. A. M.
dc.creatorGILAVERTE, S.
dc.creatorQUEIROZ, S. C. do N. de
dc.creatorASSALIN, M. R.
dc.creatorFERRACINI, V. L.
dc.creatorMONTEIRO, A. L. G.
dc.creatorREYES, F. G. R.
dc.date2017-01-17T11:11:11Z
dc.date2017-01-17T11:11:11Z
dc.date2017-01-17
dc.date2017
dc.date2017-03-02T11:11:11Z
dc.date.accessioned2026-07-07T05:03:08Z
dc.descriptionAbstract: The widespread use of moxidectin (MOX), a parasiticide used in the sheep breeding, has induced the parasite resistance in Brazilian farms. As a consequence, the farmers often increase the dose and frequency of drug utilization, and disregards safety of meat or milk. In order to establish adequate therapeutic treatment it is necessary to know the pharmacokinetics of the drug in the animal's body. Thus, high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the determination of MOX in serum lamb. Serum samples were treated with acetonitrile to precipitate proteins. A clean up by dispersive extraction in solid phase (SPE-d), using primary/secondary amine (PSA) and C18 sorbents, followed by freezing was performed. Method validation presented precision (coefficient of variation) and accuracy (recovery%) between 1.7-6.7 and 80.0-107.3%, respectively. The limit of quantification (LOQ) of the method was 2.0 ng mL-1 and a linear response was obtained over a range of 2.0 to 100 ng mL-1. This method was successfully applied to the determination of MOX in serum from suffolk lamb to evaluate the pharmacokinetic profile.
dc.identifierJournal of the Brazilian Chemical Society, Campinas, v. 28, n. 2, p. 250-256, 2017.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1060930
dc.identifier.urihttp://hdl.handle.net/123456789/478645
dc.languageeng
dc.rightsopenAccess
dc.subjectSerum lamb
dc.subjectLC-MS/MS
dc.subjectCordeiro
dc.subjectAnálise de laboratório
dc.subjectAnti-helmíntico
dc.subjectCromatografia
dc.subjectEspectrometria
dc.subjectSoro Sangüíneo
dc.subjectBlood serum
dc.subjectLambs
dc.subjectMoxidectin
dc.subjectAnthelmintics
dc.subjectPharmacokinetics
dc.subjectVeterinary drugs
dc.subjectTandem mass spectrometry
dc.subjectLiquid chromatography
dc.titleDetermination of moxidectin in serum by liquid chromatography-tandem mass spectrometry and its application in pharmacokinetic study in lambs.
dc.typeArtigo de periódico

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