Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells

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dc.creatorKubow, S.
dc.creatorIskandar, M.M.
dc.creatorMelgar-Bermudez, E.
dc.creatorSleno, L.
dc.creatorSabally, K.
dc.creatorAzadi, B.
dc.creatorHow, E.
dc.creatorPrakash, S.
dc.creatorBurgos, G.
dc.creatorFelde, T. zum
dc.date2017-09
dc.date2018-04-02T20:39:31Z
dc.date2018-04-02T20:39:31Z
dc.date.accessioned2026-06-27T18:00:50Z
dc.descriptionA dynamic human gastrointestinal (GI) model was used to digest cooked tubers from purple-fleshed Amachi and Leona potato cultivars to study anthocyanin biotransformation in the stomach, small intestine and colonic vessels. Colonic Caco-2 cancer cells and non-tumorigenic colonic CCD-112CoN cells were tested for cytotoxicity and cell viability after 24 h exposure to colonic fecal water (FW) digests (0%, 10%, 25%, 75% and 100% FW in culture media). After 24 h digestion, liquid chromatography-mass spectrometry identified 36 and 15 anthocyanin species throughout the GI vessels for Amachi and Leona, respectively. The total anthocyanin concentration was over thirty-fold higher in Amachi compared to Leona digests but seven-fold higher anthocyanin concentrations were noted for Leona versus Amachi in descending colon digests. Leona FW showed greater potency to induce cytotoxicity and decrease viability of Caco-2 cells than observed with FW from Amachi. Amachi FW at 100% caused cytotoxicity in non-tumorigenic cells while FW from Leona showed no effect. The present findings indicate major variations in the pattern of anthocyanin breakdown and release during digestion of purple-fleshed cultivars. The differing microbial anthocyanin metabolite profiles in colonic vessels between cultivars could play a significant role in the impact of FW toxicity on tumor and non-tumorigenic cells.
dc.identifierhttps://hdl.handle.net/10568/91982
dc.identifier.urihttp://hdl.handle.net/123456789/152416
dc.languageen
dc.publisherMDPI
dc.rightsOpen Access
dc.sourceKubow, S,; Iskandar, M.M.; Melgar-Bermudez, E.; Sleno, L.; Sabally, K.; Azadi, B.; How, E.; Prakash, S.; Burgos, G.; Felde, T. zum. 2017. Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells. Nutrients. (Switzerland). ISSN 2072-6643. 9(953).
dc.subjectpotatoes
dc.subjectanthocyanins
dc.subjectdigestive system
dc.subjectantioxidants
dc.subjectneoplasms
dc.subjectcytotoxicity
dc.titleEffects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells
dc.typeJournal Article

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