Development of mutants of Coniothyrium minitans with improved efficiency for control of Sclerotinia sclerotiorum.
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Coniothurium minitans (CM) is hyperparasitic to Sclerotinia sderotiorum (55), a palhogen of many economically important crops. In this paper, we describe lhe isolation of improved mutants of CM, using a UV - irradiation regime, with altered chitinase production and tolerance to high concentration of iprodione, which are effect:iveagainst Ss. Three out of the 59 mutants obtained inhibited the mycelial growth of CM. Infectivity of sclerotia by the new mutants was assayed by the plant-tissue-based system using carrot segments. More than 80%of sclerotia were colonized by lhe mutants and the wild-type CM. The mutant strains retained ability to produce significant amounts of chitinase. The mutants differed frorn their wild-type strain in appearance, morphology and sporulation. In conclusion, the results presented here provide evidence that lhe new biotypes of C. minitans are effective in controlling S.sclemtiarum.
Palabras clave
Coniothvrium minitans, Resistance, Antibiotic production, Sclerotinia sclerotiorum, Doença fúngica, Controle biológico, Fungo para controle biológico, Biological control, Antifungal agents, iprodione