Effect of age and size of hypocotyl explant on in vitro shoot regeneration in eggplant
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Society for Promotion of Horticulture
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In the present study, effect of size and age of hypocotyl explant on in vitro organogenetic responses was assessed ineggplant cv. Manjarigota. Size and age did not affect callus-initiation response, but showed marked influence on shootregeneration response. Hypocotyl explants 1.5cm long showed highest shoot regeneration response (77.4%); eitherincrease or decrease in size resulted in reduced response. Five to 15 day old hypocotyl explants showed direct shootregeneration from cut ends, whereas 20-30 day old hypocotyl explants showed indirect shoot regeneration from callusproduced on cut ends. Five day old explants were most responsive, with highest (91.23%) and thirty day old explantsleast responsive with reference to shoot regeneration response (20.85%). Shoot regeneration frequency decreasedwith increasing age, whereas shoot regeneration efficiency increased with increasing age of hypocotyl explants.
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