Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids.

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dc.contributorCarlos Alexandre Gomes Ribeiro, Universidade Federal de Viçosa; MARCOS DE OLIVEIRA PINTO, CNPMS; Talles Eduardo Ferreira Maciel, Universidade Federal de Viçosa; MARIA MARTA PASTINA, CNPMS; Everaldo Gonçalves de Barros, Universidade Católica de Brasília; CLAUDIA TEIXEIRA GUIMARAES, CNPMS.
dc.creatorRIBEIRO, C. A. G.
dc.creatorPINTO, M. de O.
dc.creatorMACIEL, T. E. F.
dc.creatorPASTINA, M. M.
dc.creatorGOMES, E. G. de
dc.creatorGUIMARAES, C. T.
dc.date2017-11-10T23:21:31Z
dc.date2017-11-10T23:21:31Z
dc.date2017-11-10
dc.date2017
dc.date2017-11-10T23:21:31Z
dc.date.accessioned2026-07-07T05:21:28Z
dc.descriptionThe development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.
dc.identifierScientia Agricola, Piracicaba, v. 74, n. 2, p. 163-168, 2017.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1079407
dc.identifier10.1590/1678-992x-2016-0079
dc.identifier.urihttp://hdl.handle.net/123456789/488445
dc.languageeng
dc.rightsopenAccess
dc.subjectGenotipagem
dc.subjectCaracterização genética
dc.subjectZea mays
dc.subjectVariedade
dc.titleUniversal tail sequence-SSR applied to molecular characterization of tropical maize hybrids.
dc.typeArtigo de periódico

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