CYTOGENETIC CHARACTERIZATION OF NEW WILD CLONES OF THE Saccharum COMPLEX
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Instituto Nacional de Ciencias Agrícolas
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In order to confirm taxonomic identification, a chromosomal count of new wild clones of Saccharum complex was performed and Giemsa C-banding technique was adapted to develop the use of polymorphism patterns as possible chromosome markers. Clones, cytogenetically characterized through these techniques, constituted new accessions representative of Erianthus and Saccharum genera. Twelve chromosomic patterns were revealed among the C-banding and ten of these presented bands at different positions lengthwise the chromosome. Five chromosomic patterns separated three clonal groups: Erianthus, Saccharum and hybrid clones. To determine the hybrid origin of ECL 5-4, ECL 1-29 and ECL 1-31 clones, a Factor Analysis of Correspondence (FAC) was performed. To confirm the discriminative value of the five patterns associated to clonal groups and their within-group uniformity, a test of independence (P<0.01) was used. Effectiveness of use of possible cytogenetical markers in the taxonomic identification was demonstrated.
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