A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium.

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dc.contributorDANIEL DIAS RUFINO ARCANJO, UNIVERSIDADE FEDERAL DO PIAUÍ
dc.contributorANDREANNE GOMES VASCONCELOS, UNIVERSIDADE FEDERAL DO PIAUÍ
dc.contributorSIMÓN GABRIEL, AARHUS UNIVERSITY
dc.contributorJOILSON RAMOS JESUS, UNIVERSIDADE FEDERAL DO PIAUÍ
dc.contributorLUCIANO PAULINO DA SILVA, CENARGEN
dc.contributorOSMINDO RODRIGUES PIRES JÚNIOR, UNIVERSIDADE DE BRASÍLIA
dc.contributorCLAUDIO MIGUEL COSTA-NETO, UNIVERSIDADE DE SÃO PAULO
dc.contributorEDUARDO BRANDT OLIVEIRA, UNIVERSIDADE DE SÃO PAULO
dc.contributorLUDOVICO MIGLIOLO, UNIVERSIDADE CATÓLICA DE BRASÍLIA
dc.contributorOCTÁVIO LUIZ FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA
dc.contributorCAROLINA BARALDI ARAÚJO RESTINI, UNIVERSIDADE DE RIBEIRÃO PRETO
dc.contributorMICHELE PAULO, UNIVERSIDADE DE SÃO PAULO
dc.contributorLUSIANE MARIA BENDHACK, UNIVERSIDADE DE SÃO PAULO
dc.contributorMARCELO PORTO BEMQUERER, CENARGEN
dc.contributorALDEIDIA PEREIRA OLIVEIRA, UNIVERSIDADE FEDERAL DO PIAUÍ
dc.contributorULF SIMONSEN, AARHUS UNIVERSITY
dc.contributorJOSÉ ROBERTO DE SOUZA DE ALMEIDA LEITE, UNIVERSIDADE FEDERAL DO PIAUÍ.
dc.creatorARCANJO, D. D. R.
dc.creatorVASCONCELOS, A. G.
dc.creatorCOMERMA-STEFFENSEN, S. G.
dc.creatorJESUS, J. R.
dc.creatorSILVA, L. P. da
dc.creatorPIRES JÚNIOR, O. R.
dc.creatorCOSTA-NETO, C. M.
dc.creatorOLIVEIRA, E. B.
dc.creatorMIGLIOLO, L.
dc.creatorFRANCO, O. L.
dc.creatorRESTINI, C. B. A.
dc.creatorPAULO, M.
dc.creatorBENDHACK, L. M.
dc.creatorBEMQUERER, M. P.
dc.creatorOLIVEIRA, A. P.
dc.creatorSIMONSEN, U.
dc.creatorLEITE, J. R. de S. de A.
dc.date2018-09-01T00:44:46Z
dc.date2018-09-01T00:44:46Z
dc.date2015-12-15
dc.date2015
dc.date2018-09-01T00:44:46Z
dc.date.accessioned2026-07-07T11:51:51Z
dc.descriptionProline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases.
dc.identifierPlos One, v. 10, dez. 2015. (Open Access)
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1031700
dc.identifier10.1371/journal.pone.0145071
dc.identifier.urihttp://hdl.handle.net/123456789/515452
dc.languageeng
dc.rightsopenAccess
dc.subjectProline-rich oligopeptides
dc.subjectBrachycephalus ephippium
dc.subjectsecretion
dc.titleA novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium.
dc.typeArtigo de periódico

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